Browse > Article

Hypoglycemic Effect of Polysaccharide Isolated from Eclipta alba L.  

Woo, Eun-Rhan (College of Pharmacy, Chosun University)
Lee, Dong-Haeng (College of Pharmacy, Chosun University)
Moon, Young-Hee (College of Pharmacy, Chosun University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 246-249 More about this Journal
Abstract
The major polysaccharide, named EAP, was purified from the aerial parts of Eclipta alba by Sepharose CL-2B ion exchange chromatography and recycling HPLC. The molecular weight of EAP was estimated to be 20911.9 D by MALDI-TOF MS. In addition, the sugar composition was determined to be arabinose (23.6%), mannose (24.8%), galactose (12.3%), and glucose (41.3%), respectively, by GC analysis. The EAP decreased the blood sugar level, which was induced by alloxan in rats, dose dependently.
Keywords
Eclipta alba; polysaccharide; hypoglycemic activity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hikino, H., Takahashi, M., Otake, K., and Konno, C. (1986) Isolation and hypoglycemic activity of eleutherans A, B, C, D, E, F, and G: Glycans of Eleutherococcus senticosus roots. J. Nat. Prod. 49: 293-297   DOI   PUBMED
2 Singh, B., Saxena, K., Chandan, B., Agarwal, S., Bhatia, M., and Anand, K. (1993) Hepatoprotective effect of ethanol extract of Eclipta alba on experimental liver damage in rats and mice, Phytother. Res. 7: 154-158   DOI   ScienceOn
3 Karas, M. and Hillenkamp, F. (1988) Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons, Anal. Chem. 60: 2299-2307   DOI   ScienceOn
4 Chaplin, M.F. (1994) In carbohydrate analysis, Oxford University Press, p. 6, 32
5 Lee, E.B. and Kim, O.K. (1993) Antihyperglycemic constituents of Aralia elata root Bark (I), Kor. J Pharmacogn. 24: 213-218
6 Bohlmann, E and Zdero, C. (1970) Uber die inhaltsstoffe aus Eclipta erectra L. Chem. Ber. 103: 834-841   DOI
7 배기환(2000) 한국의 약용식물, 교학사, 서울 p.502
8 Zhu, YP (1998) Chinese Materia Medica, Harwood academic publishers, The Netherlands, p.646
9 Medina, ES., Gamez, MJ., Jimenez, I., Osuna, I.I., and Zarzuelo, A. (1994) Hypoglycemic activity of Juniper Berries. Planta Med. 60: 197-203   DOI   ScienceOn
10 Krishnaswamy, N.R., Seshadri, T.R., and Sharma, B.R. (1966) The structure of a new polythienyl from Eclipta alba. Tetrahedron. Lett. 35: 4227-4231
11 Ohno, N., Suzuki, I., Sato, K., Oikawa, S., Miyazaki, T., and Yadomae, T. (1985) Purification and structural characterization of an antitumor $\beta$-1,3-glucan isolated from hot water extract of the fruit body of cultured Grifola frondosa. Chem. Pharm. Bull. 33: 4522-4527   DOI
12 Zhang, Z., Kiyohara, H., Matsumoto, T., and Yamada, H. (1997) Fractionation and chemical properties of immunomodulating polysaccharides from roots of Dipsacus asperoides. Planta Med. 63: 393-398   DOI   ScienceOn
13 李時珍(1975) 圖解 本草綱目, 高文社, 서울 p.630
14 Chihara, G., Hamuro, J., Maeda, Y., Artai, Y., and Fukuoka, F. (1970) Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes, an edible mushroom. Cancer Res. 30: 2776-2781
15 李昌福(1982) 大韓植物圖鑑, 鄕文社, 서울 p.763
16 Bhargava, K.K., Krishnaswamy, N.R., and Seshadri, T.R. (1972) Desmethylwedelolactone glucoside from Eclipta alba leaves, Indian J. Chem. 10: 810-811
17 Lukens, F.D.W. (1948) Alloxan diabetes, Physiol. Rev. 28: 304-330
18 Kato, A. and Miura, T. (1994) Hypoglycemic action of the rhizomes of Polygonatum officinale in normal and diabetic mice. Planta. Med. 60: 201-206   DOI   ScienceOn
19 Bhrgava, K.K., Krishnaswamy, N.R., and Seshadri, T.R. (1970) Isolation of desmethylwevelolactone & its glucoside from Eclipta alba, Indian J. Chem. 8: 664-665   DOI   ScienceOn
20 Redier, D.D., Brown, R.S., Weingerger, S., Kenny, J., and Bailey, J. (1998) Unknwn peptide sequencing using matrixassisted laser desorption/ionixation and in source decay. Anal. Chem. 70: 1214-1222   DOI   ScienceOn
21 Srivastava, R. and Kulshreshtha, K.K. (1989) Bioactive polysaccharides from plants. Phytochemistry 28: 2877-2902   DOI   ScienceOn