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Effects of Chelidonine on L-DOPA-Induced Cytotoxicity in PC12 Cells  

Kim, Yu-Mi (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Lee, Jae-Joon (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Jin, Chun-Mei (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Yang, Yoo-Jung (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Kang, Min-Hee (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 242-245 More about this Journal
Abstract
The effects of chelidonine, a benzophenanthridine isoquinoline alkaloid, on L-DOPA-induced cytotoxicity in PC12 cells were investigated. The treatment of PC12 cells with chelidonine $(1-4\;{\mu}M)$ decreased dopamine content in a dose-dependent manner (30.2% inhibition at $4\;{\mu}M)$. Chelidonine was not cytotoxic up to $4\;{\mu}M)$. However, chelidonine at concentrations higher than $5\;{\mu}M$ caused a cytotoxicity in PC12 cells. L-DOPA at concentrations higher than $50\;{\mu}M$ led to cell damage by oxidative stress in PC12 cells. Chelidonine at non-cytotoxic concentration ranges of $1-4{\mu}M$ aggravated L- DOPA $(20-50\;{\mu}M)$-induced cytotoxicity in PC12 cells. The L-DOPA-induced cytotocxicity was synergistically stimulated by chelidonine at concentrations grader than $5\;{\mu}M$. These data demonstrate that chelidonine exacerbates L-DOPA-induced cytotoxicity. Therefore, it is proposed that the long-term L-DOPA therapeutic patients with chelidonine may need to be checked for the adverse symptoms.
Keywords
Chelidonine; L-DOPA-induced cytotoxicity; PC12 cells;
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