Browse > Article

Growth Inhibitory Effects of Sesamolin from Sesame Seeds on Human Leukemia HL-60 Cells  

Kim, Kwan-Su (Dept. of Medicinal Plant Resources, School of Biotechnology & Resources, Mokpo National University)
Kang, Sam-Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University)
Ryu, Su-Noh (Dept. of Agricultural Science, Korea National Open University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 237-241 More about this Journal
Abstract
This study was carried out to test the growth inhibitory effects of sesamolin obtained from sesame seeds. Sesamolin inhibited the growth of human leukemia HL-60 cells in cultures and the synthesis of macromolecules in dose- and time-dependent manners. Sesamolin in the $60{\sims}100\;{\mu}g/ml$ range was cytostatic. At concentrations greater than $200\;{\mu}g/ml$ sesamolin was cytocidal to HL-60 cells and at $60\;{\mu}g/ml$ inhibited the synthesis of DNA, RNA and protein in HL-60 cells by 35.1, 6.1, and 5.3%, whereas at $200\;{\mu}g/ml$ these inhibitions were 86.8%, 81.5% and 96.7%, respectively. The inhibitory effect of sesamolin on DNA synthesis was irreversible.
Keywords
Sesame (Sesamum indicum); sesamolin; growth inhibitory effects; human leukemia HL-60 cells;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fukuda, Y., Nagata, M., Osawa, T., and Namiki, M. (1986) Contribution of lignan analogues to antioxidative activity of refined unroasted sesame seed oil. J. Amer. Oil Chem. Soc. 63: 1027-1031   DOI
2 Asami, S., Akimoto, K., Abe, K., Akamatsu, T., Konishi, K., Shimizu, S., Sugano, M., and Yamada, H. (1993) Antioxidant activity of sesamin on NADPH-dependent lipid peroxidation in liver microsomes. Nippon Nogeikagaku Kaishi 67: 265
3 Tricker, D. and Shklar, G. (1987) Prevention by vitamin E of experimental oral carcinogenesis. J. Nutr. Cancer 16: 43-52
4 Weiss, S. G., Tin-Wa, M., Perdue, R. E., Jr., and Famsworth, N. R. (1975) Potential anticancer agents. II. Antitumor and cytotoxic lignan from Linum album (Linaceae). J. Pharm. Sci. 64: 95-98   DOI   PUBMED
5 Yamashita, K., Nohara, Y., Katayama, K., and Namiki, M. (1992) Sesame seed lignans and $\alpha$-tocopherol act synergistically to produce vitamin E activity in rats. J. Nutr. 122: 2440-2446
6 Katsuzaki, H., Kawakishi, S., and Osawa, T. (1994) Sesaminol glucosides in sesame seeds. Phytochemistry 35: 773-776   DOI   ScienceOn
7 Osawa, T. (1992) Phenolic antioxidants in dietary plants as antimutagens. In Huang, M.-T., Ho, C.-T. and Lee, C. Y. (ed.), Phenolic Compounds in Food and their Effects on Health II: Antioxidants & Cancer Prevention, 135-149. ACS. Washington, DC.
8 Akimoto, K., Kitagawa, Y., Akamatsu, T., Hirose, N., Sugano, M., Shimizu, S., and Yamada, H. (1993) Protective effects of sesamin against liver damage cause by alcohol or carbon tetrachloride in rodents. Ann. Nutr. Metab. 37: 218-224   DOI   ScienceOn
9 Miyahara, Y., Hibasami, H., Katsuzaki, H., Imai, K., and Komiya, T. (2001) Sesamolin from sesame seed inhibits proliferation by inducing apoptosis in human lymphoid leukemia Molt 4B cells. Int. J. Mol. Med. 7: 369-371
10 Ryu,S. N., Ho,C.-T., and Osawa, T. (1998) Highperformance liquid chromatograpic determination of lignan glycosides in some varieties of sesame. J. Food Lipid 5: 17-28   DOI   ScienceOn
11 Hirose, N., Doi, F., Ueki, T., Akazawa, K., Chijiiwa, K., Sugano, M., Akimoto, K, Shimizu, S., and Yamada, H. (1992) Suppressive effect of sesamin against 7,12-dimethylbenz[$\alpha$]-anthracene induced rat manunary carcinogenesis. Anticancer Res. 12: 1259-1266
12 Harman, D. (1969) Dimethy1benzanthracene induced cancer: Inhibiting effect of dietary vitamin E. Clin. Res. 17: 125
13 Ryu, S. N., Lee, J. I., and Kang, S. S. (1994) Isolation, identification and quantitative analysis of antioxidants in sesame seed. RDA J. Agricul. Sci. 36: 122-126
14 Hirose, N., Inoue, T., Nishihara, K., Sugano, M., Akimoto, K., Shimizu, S., and Yamada, H. (1991) Inhibition of cholesterol absorption and synthesis in rats by sesamin. J. Lipid Res. 32: 629-638
15 Fujiyama-Fujiwara, Y., Umeda-Sawada, R., Kuzuyama, M., and Igarashi, O. (1995) Effects of sesamin on the fatty acid composition of the liver of rats fed N-6 and N-3 fatty acid riched diet. J. Nutr. Sci. Vitaminol. 41: 217-225   DOI   ScienceOn
16 Fukuda, Y., Osawa, T., Kawakishi, S., and Namiki, M. (1994) Chemistry of lignan antioxidants in sesame seed and oil. In Ho, C.-T, Osawa, T., Huang, M.-T, and Rosen, R. T. (ed.), Food Phytochemicals for Cancer Prevention II: Teas, Spices, and Herbs, 264-274. ACS. Washington, DC
17 Fukuda, Y., Osawa, T., Namiki, M., and Ozaki, T. (1985) Studies on antioxidative substances in sesame seed. Agric. Biol. Chem. 49: 301-306   DOI
18 Shimizu, S., Akimoto, K., Shinmen, Y., Kawashima, H., Sugano, M., and Yamada, H. (1991) Sesamin is a potent and specific inhibitor of $\Delta^5$-desaturase in polyunsaturated fatty acid biosynthesis. Lipids 26: 512-516   DOI   ScienceOn
19 Sugano, M., Inoue, T., Koba, K., Yoshida, K., Hirose, N., Shinmen, Y., Akimoto, K., and Amachi, T. (1990) Influence of sesame lignans on various lipid parameters in rats. Agric. Biol. Chem. 54: 2669-2673   DOI
20 Fujiyama-Fujiwara, Y., Umeda, R., and Igarashi, O. (1992) Effect of sesamin and curcumin on $\Delta^5$-desaturationand chain elongation of polyunsaturated fatty acid metabolism in primary cultured rat hepatocytes. J. Nutr. Sci. Vitaminol. 38: 353-363
21 King, M. M. and McCay, P. B. (1983) Modulation of tumor incidence and possible mechanism of inhibition of manunary carcinogenesis by dietary antioxidants. Cancer Res. 43: 2485s-2490s