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In vitro Antiinflammatory Activity of Amygdalin in Murine Macrophage Raw 264.7 Cells  

Shin, Kyung-Min (College of Pharmacy, Kyung Hee University)
Park, Young-Mi (College of Pharmacy, Kyung Hee University)
Kim, In-Tae (College of Pharmacy, Kyung Hee University)
Hong, Seon-Pyo (College of Pharmacy, Kyung Hee University)
Hong, Jung-Pyo (College of Dentistry, Kyung Hee University)
Lee, Kyung-Tae (College of Pharmacy, Kyung Hee University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 223-227 More about this Journal
Abstract
In the present study, anti-inflammatory activity of amygdalin isolated from persicae Semen have been evaluated on lipopolysaccharide (LPS)-induced release of nitric oxide (NO), prostaglandin $E_2\;(PGE_2)$ and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) by the macrophage RAW 264.7 cells. Amygdalin significantly inhibited generation of NO and $TNF-{\alpha}$ on LPS-stimulated RAW264.7 cells in a concentration-dependent manner. Consistent with these observations, the expression of inducible NO synthase (iNOS) enzyme was also inhibited by amygdalin in a concentration-dependent manner. However, amygdalin did not show any influence on the synthesis of $PGE_2$ and the expression of COX-2. Thus, this study suggests that amygdalin-mediated inhibition of iNOS expression, and $TNF-{\alpha}$ release may be one of the mechanisms responsible for the anti-inflammatory effects of Persicae Semen.
Keywords
Amygdalin; nitric oxide; RAW 264.7; iNOS; $TNF-{\alpha}$; Persicae Semen;
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