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The Effect of Korean Mistletoe Extract M11C (Non-Lectin Components) on the Tumor Necrosis $Factor-{\alpha}$ Secretion from Mouse Splenic Macrophages and on the Inhibition of Sarcoma 180-Induced Tumor Growth in Mice  

Sung, Ki-Tai (Department of Immunology, College of Medicine, Konkuk University and Department of Oriental Medicine, College of Oriental Medicine, Semyung University)
Kang, Tae-Bong (Department of Immunology, College of Medicine, Konkuk University and The Institute for Biomedical Research, Handong University)
Jun, Myung-Ha (Department of Immunology, College of Medicine, Konkuk University)
Chang, Sung-Ho (Department of Immunology, College of Medicine, Konkuk University)
Lee, Jun-Ho (Department of Immunology, College of Medicine, Konkuk University)
Kim, Jong-Bae (The Institute for Biomedical Research, Handong University)
Choi, Wahn-Soo (Department of Immunology, College of Medicine, Konkuk University)
Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University)
Seong, Nak-Sul (National Crop Experiment Station, RDA)
Lee, Sung-Tae (Department of Orthopaedic Surgery, College of Medicine, Konkuk University)
Sung, Hyun-Jea (Department of Oriental Medicine, College of Oriental Medicine, Semyung University)
Her, Erk (Department of Immunology, College of Medicine, Kunkuk University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 210-217 More about this Journal
Abstract
Korean mistletoe (Viscum album) extract has been found to posses immunostimulatory activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract might activate mouse splenic macrophages to produce tumor necrosis $factor-{\alpha}\;(TNF-{\alpha}$) and might play a role in anticancer. To know the effect of M11C on the production of $TNF-{\alpha}$, the splenic macrophages were treated by the M11C, and then collected the supernatant (M11C stimulated splenic macrophage-conditioned media; MSCM). MSCM was analyzed for the $TNF-{\alpha}$ secretion by means of ELISA and immunoblotting, and mRNA expression was analyzed by RT-PCR. The S-180 murine sarcoma model was established to know the effect of M11C on the inhibition of tumor growth. M11C had the effect of $TNF-{\alpha}$ production from splenic macrophages performed by ELISA technique. This ELISA data was reconfirmed by immunoblotting assay. The effects of M11C on the expression of $TNF-{\alpha}$ mRNA from the macrophages was also shown. M11C also had the inhibitory effect of S-180 tumor growth. These data suggest that Korean mistletoe extract M11C may be used for an immunomodulator.
Keywords
Korean mistletoe; non-lectin components; splenic macrophages; $TNF-{\alpha}$ release; $TNF-{\alpha}$ expression; anti-tumor growth;
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