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Effects of Liriodenine on Dopamine Biosynthesis in PC12 Cells  

Jin, Chun-Mei (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
Lee, Jae-Joon (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
Yin, Shou-Yu (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
Kim, Yu-Mi (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
Kim, Young-Kyoon (College of Forest Science, Kookmin University)
Rhu, Shi-Yong (Korea Research Institute of Chemical Technology)
Lee, Myung-Koo (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.1, 2003 , pp. 55-59 More about this Journal
Abstract
The effects of liriodenine, an aporphine isoquinoline alkaloid, on dopamine content in PCl2 cells were investigated. Treatment of PC12 cells with liriodenine decreased dopamine content in a dose-dependent manner (33.6% inhibition at $10\;{\mu}M$ for 12 h). The $IC_{50}$ in value of liriodenine was $8.4\;{\mu}M$. Dopamine content decreased at 3 h and reached a minimal level at 12 h after the exposure to liriodenine. Under these conditions, the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase were also inhibited at $10\;{\mu}M$ of liriodenine by 10.1% and 20.2% relative to control, respectively. In addition, liriodenine inhibited the increase in dopamine content induced by L-DOPA Treatments $(50-100\;{\mu}M)$ in PC12 cells. These results suggest that liriodenine inhibited dopamine biosynthesis and L-DOPA-induced increase in dopamine content by reducing the activities of tyrosine hydroxylase and aromatic L- amino acid decarboxylase in PC12 cells.
Keywords
Liriodenine; dopamine content; tyrosine hydroxylase; aromatic L-amino acid decarboxylase; PC12 cells;
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