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Screening of Monocyte Chemoattractant Protein-1-Induced Chemotaxis Inhibitors from Medicinal Herbs  

Lee, Seung-Woong (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kwon, Oh-Eok (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Chung, Mi-Yeon (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Ho (College of Pharmacy, Chungnam National University)
Lee, Hyun-Sun (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Kook (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Rho, Mun-Chual (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.4, 2002 , pp. 352-358 More about this Journal
Abstract
Blood monocytes are the precursors for the lipid-laden foam cells of early atherosclerotic lesions. Monocyte chemoattractant protein-1(MCP-1), a CC chemokine, and chemokine receptor 2 (CCR2) play a crucial role in the recruitment of monocytes to the vascular lesion. Using the human monocyte THP-1 cell line, we investigate the inhibitory effects of methanol extracts of 127 medicinal herbs on MCP-1 induced chemotaxis. Seven kinds of methanol extracts of medicinal herbs showed above 40% inhibitory effect with the concentration of $25{\mu}g/ml$. They were divide three fractions of $CHCI_3$, BuOH, $H_2O$ to use solvent partition. Among them, butanol extract of Junci Medulla and $CHCI_3$ extract of Clematidis Radix are showed significant inhibitory activities (above 50% inhibition) at the same concentration.
Keywords
Medicinal herb; Chemotaxis; Monocyte chemoattractant protein-1;
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