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Inhibitory Effects of Natural Plant Extracts on ICAM-1/LFA-1 Mediated Adhesion of HL-60 Cells  

Kwon, Oh-Eok (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Lee, Seung-Woong (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Chung, Mi-Yeon (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Ho (College of Pharmacy, Chungnam National University)
Lee, Hyun-Sun (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Kook (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Rho, Mun-Chual (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.4, 2002 , pp. 343-351 More about this Journal
Abstract
Atherosclerosis is a progressive disease characterized by the accumulation of lipids and fibrous elements in the arteries. Monocyter/macrophages are involved in many aspects of the development of atherosclerotic plaques. It is known that the intercellular adhesion molecule-1(ICAM-1) expressed preferentially on endothelial cells of atherosclerotic plaque, promotes local adhesion and transendothelial migration of monocytes, neutrophils, and lymphocytes. Using the human promyelocytic leukemia HL-60 cell line, we investigated the inhibitory effects of methanol extracts of 175 natural plants on ICAM-1/LFA-1 mediated cell adhesion. Eight kinds of methanol extracts of tested plants inhibited PMA-induces homotypic aggregationof HL-60 cells without cytotoxicity at the concentration of $6.25\;{\mu}g/ml$. They were divided two fractions of $CHCI_3$ and $H_2O$ to use solvent partition. Among them, $CHCI_3$ extract $(1.0\;{\mu}g/ml)$ of Saururus chinensis and Chloranthus japonicus singificantly inhibited aggregation of HL-60 cells without cytotoxicity, respectively.
Keywords
Cell adhesion molecules; ICAM-1; Atherosclerosis; Plant extracts; HL-60 cells;
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1 Rothlein, R., Dustin, M. L., Marlin, S. D. and Spnnger, T. A. (1986) A human intercellular adhesion molecule-1 (ICAM-1) distinct from LFA-l. J. Immunol. 137: 1270-1274
2 Springer, T. A. (1990) Adhesion receptors of the immune system. Nature 346: 425-434   DOI   PUBMED   ScienceOn
3 Dustin, M. L. and Springer, T. A. (1989) T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-l. Nature 341: 619-624   DOI   ScienceOn
4 Thomas, J. and DeGraba, M. D. (1997) Expression of inflammatory mediators and adhesion molecules in human atheroscleotic plaque. Neurology 49: 15-19
5 Collins, S. J., Gallo, R. C. and Gallagher, R. E. (1977) Continuous growth and differentiation of human myeloid leukemic cells in suspension culture. Nature 270: 347-349   DOI   ScienceOn
6 Katagiri, K., Katagiri, T., Koyama, Y., Morikawa, M., Yamamoto, T. and Yoshida, T. (1991) Expression of src family genes during monocytic differentition of HL-60 cells J. Immunol. 146: 701-707
7 Nishikawa, K., Guo, Y. J., Miyasaka, M., Tamatani, T, Collins, A. B., Sy, M. S., McCluskey, R. T. and Andres, G. (1993) Antibodies to intercellular adhesion molecule 1/lymphocyte function-associated antigen 1 prevent crescent formation in rat autoimmune glomenrulonephritis. J. Exp. Med. 177: 667-77   DOI   ScienceOn
8 Hubbard, A. K. and Rothlein, R. (2000) Intercellular adhesion molecule-1 (ICAM-l) expression and cell signaling cascades. Free Radic. Biol. Med. 28: 1379-1386   DOI   ScienceOn
9 Collins, S. J. (1987) The HL-60 promyelocytic leukemia cell line proliferation, differentiation, and cellular oncogene expression. Blood 70: 1233-1244
10 Rothlein, R. and Springer, T. A. (1986) The requirement for lymphocyte function-associated antigen 1 in homotypic leukocyte adhesion stimulated by phorbol ester. J. Exp. Med. 163: 1132-1149   DOI   ScienceOn
11 Patel, S. S., Thiagarajan, R., Willerson, J. T. and Yeh, E. T. (1998) Inhibition of alpha4 integrin and ICAM-l markedly attenuate macrophage homing to atherosclerotic plaques in ApoE-deficient mice. Circulation 97: 75-81   DOI   ScienceOn
12 Kobayashi, Y., Kawai, K., Honda, H., Tomida, S., Niimi, N., Tamatani, T., Miyasaka, M. and Yoshikai, Y. (1995) Antibodies against leukocyte function-associated antigen-1 and against intercellular adhesion molecule-1 together suppress the progression of experimental allergic encephalomyelitis. Cell Immunol. 164: 295-305Nagase, T., Fukuchi, Y., Dallaire, M. J., Martin, J. G. and Ludwig, M. S. (1995) In vitro airway and tissue response to antigen in sensitized rats. Role of serotonin and leukotriene D4. Am. J. Respir. Crit. Care Med. 152: 81-86텝돀颲⨀𪒖⨀𪒖⨀頳⨀ᤠ돐ࡗ잖⨀ࡗ잖⨀쀳⨀덐퀳⨀Ѐ㈰〸ࠀ〰〰〰㈸ࠀ〰〰〰〵ᄀ䍓卐䉑弲〰㡟瘲㡮㔂2㌌ìꂜ㈸谵렀ఀ䥇砀刺￿ｿ䥇騀￿首䥇鬀￿骖䥇鰀ﯿ羚᪛䥇鴀￿￿䥇뤀￿７䩇먀肛᠛䩇묀Ȁ€䩇츀耘骘䩇케￿７ᮚ䩇퀀ࠀ䭇ࠀ肘鬙䭇８᨜䭇ഀ€䭇ऀ鲖䭇￿￿䭇羙ᰛ䱇ﯿ￿ｿ䱇切Ȁš鮘䱇ff﷿ｿ墙兇ﰀ؀ᬜ峝呇ﴀ硌⺬闷浇︀处돀ᢰಗ⨀⁊렼惫ಗ⨀㄄돐胫ಗ⨀胡ಗ⨀Ȁﰀﴀ︀鰀鴀뤀먀묀츀케퀀切ff﷿ｿ墙兇؀ᬜ峝呇硌⺬闷浇￿骖䥇ﯿ羚᪛䥇￿￿䥇￿７䩇肛᠛䩇Ȁ€䩇耘骘䩇￿７ᮚ䩇ࠀ䭇ࠀ肘鬙䭇８᨜䭇ഀ€䭇ऀ鲖䭇￿￿䭇羙ᰛ䱇ﯿ￿ｿ䱇Ȁš鮘䱇ༀ   DOI   ScienceOn
13 Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63   DOI   PUBMED   ScienceOn
14 Christoper, K. G. and Joseph, L. W. (2001) Atherosclerosis: The road ahead. Cell 104: 503-516   DOI   ScienceOn
15 Nagase, T., Fukuchi, Y, Dallaire, M. J., Mardn, J. G. and Ludwig, M. S. (1995) In vitro airway and tissue response to antigen in sensitized rats. Role of serotonin and leukotriene D4. Am. J. Respir. Crit. Care Med. 152: 81-86   DOI   ScienceOn
16 Das, D., Pintucci, G. and Stem, A. (2000) MAPK-dependentexpression of p21^W^A^F and p27^k^i^p^l in PMA-induced differentiation of HL-60 cells. FEBS Lett. 472: 50-52   DOI   ScienceOn
17 Ross, R. (1995) Cell biology of atherosclerosis. Annu. Rev. Physiol. 57 : 791-804   DOI   PUBMED   ScienceOn