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Effect of Silk Fibroin Hydrolysate on Adipocyte Metabolism in db/db Mice  

Hong, Seong-Eui (Department of Biotechnology, Graduate School, Handong University)
Park, Kum-Ju (Department of Biotechnology, Graduate School, Handong University)
Suh, Byung-Sun (School of Bioscience and Food Technology, Handong University)
Do, Myoung-Sool (School of Bioscience and Food Technology, Handong University)
Hyun, Chang-Kee (School of Bioscience and Food Technology, Handong University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.4, 2002 , pp. 312-318 More about this Journal
Abstract
Effect of the acid hydrolysate of silk fibroin on obesity was investigated in obese(057BL/KsJ-db/db) mice. After 8 weeks feeding of 1%(w/w) or 3%(w/w) fibroin hydrolysate, the extents of reduction in body weight were significantly higher than that of obese control. The weight reduction in female mice was higher than that in male mice. Plasma leptin in male mice increased up to 1.8-fold higher level than obese control by feeding hydrolysate. In case of female mice, however, it rather decreased with increased feeding concentration of hydrolysate. From the results of high glycine and serine contents of peptide fractions contained in fibroin hydrolysate, it was inferred that fibroin peptides might affect xylosyltransferase(XT) activity on chondroitin sulfate synthesis causing to change susceptibility of adipocytes to hormones such as insulin followed by the reduced leptin synthesis in female mice. The result of the higher lipolysis in hydrolysate-fed group than obese control indicated that the reduction in body weight was due to the increased lipolytic activities in male and female mice in common.
Keywords
Silk fibroin hydrolysate; Adipocyte; Leptin; Glycerol; Weight reduction;
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