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Effect of Quercetin on Melanogenesis in Melan-a Melanocyte Cells  

Choi, Won-Hyung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
Chun, Hyun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.3, 2002 , pp. 245-251 More about this Journal
Abstract
Flavonoid seems to have various biological effects. Quercetin is a kind of natural plant flavonoids and has multiple biological effects such as antioxidant, antimutagenic and anticarcinogenec agent. Melanogenesis is a physiological process resulting in the synthesis of melanin pigments, which play a crucial protective role against skin photocarcinogenesis. This present study was designed to investigate effect of quercetin on proliferation and melanogenesis in Melan-a melanocyte cells. After 48h treatment of cells with quercetin, the cells exhibited a dose-dependent inhibition in their proliferation without apoptosis. Therefore, thε growth retardation by the extract may be due to the cell arrest or cell differentiation. We also investigated the effect of quercetin on melanogenesis of this cells. Melan-a melanocyte cells were grown for 48h in the presence of $0.01-60\;{\mu}g/ml$ quercetin and the total melanin content and activity of tyrosinase were measured. Quercetin stimulated melanization of the cells in low concentrations $(0.01-1.0\;{\mu}g/ml)$, whereas it inhibited melanization in high concentrations $(5.0-30\;{\mu}g/ml)$. It was observed that quercetin differently regulates melanogenesis of Melan-a melanocyte cells dependent on Its concentrations.
Keywords
Quercetin; Melan-a melanocyte cells; melanogenesis; tyrosinase activity; melanin content;
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