Browse > Article

Determination of Coptidis Rhizoma Alkaloids in Preparations by Spectrophotometric Method  

Lim, So-Yun (College of Pharmacy, Woosuk University)
Kim, Sung-Eun (College of Pharmacy, Woosuk University)
Kim, Dae-Keun (College of Pharmacy, Woosuk University)
Shin, Tae-Yong (College of Pharmacy, Woosuk University)
Lim, Jong-Pil (College of Pharmacy, Woosuk University)
Eom, Dong-Ok (College of Pharmacy, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.3, 2002 , pp. 182-186 More about this Journal
Abstract
The Coptidis rhizoma is known for containing protoberberine alkaloids. Berberine, coptisine and palmatine are the major constituents of protoberberine alkaloids. The alkaloids were isolated and determined by forming complex compounds from Coptidis rhizoma in preparation I(Sam-Hwang-Sa-Sim-Tang) and II(Hwang-Ryen-Tang). For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex compound ion. The absorbance of alkaloidal complex compounds in l.2-dichloroethane solution was measured at 625 nm. Calibration curve for the alkaloids isolated from Coptidis rhizoma was linear over the concentration range of 0.2-0.3 mg/ml. The method was proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Coptidis rhizoma preparation I and II.
Keywords
Sam-Hwang-Sa-Sim-Tang; Hwang-Ryen-Tang; Coptidis rhizoma alkaloids; spectrophotometric method; determination;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Sawada, T., Yamahara, J. and Morika, E. (1973) The Assay of Berberine-Alkaloids by CM-Sephadex(H); The Quantita-tive Analysis of Berbenne in Crude Drug Preparations.Syoyakugaku Zasshi 27: 106-109
2 Misaki, T., Sagara, K., Ojima, M., Kakizawa, S., Oshima, T. and Yosihzawa, H. (1982) Simultaneous Determination of Berbeiine, Palmatine and Coptisine m Crude Drugs and Oriental Pharmaceutical Preparations by lon-Pair High-Performance Liquid Chromatography. Chem. Pharm. Bull. 30: 354-357   DOI   ScienceOn
3 Okamura, N., Miki, H., Ishida, S., Ono, H., Yano, A., Tanaka, T., Ono, Y. and Yagi, A. (1999) Simultaneous Determinadon of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Perfonnance Liquid Chromatography. Biol. Pharm. Bull. 22: 1015-1021   DOI   ScienceOn
4 Chen, Y. R., Wen, K. C. and Her, G. R. (2000) Analysis of copdsine, berbeiine and palmatine in adulterated Chinese medicine capillary electrophoresis-electrospray ion trap mass spectrometry. J. Chmmatography A 866: 273-280   DOI   ScienceOn
5 Sakai, T. (1979) Applicadon of Thennochromism in Spectro-photometric Analysis: Selective Determination of Berberine in Pharmaceuticals by Solvent Extraction. J. Pharm. Sci. 68: 875-877   DOI   PUBMED
6 El-Masry, S., Korany, M. A. and Abou-Donia, A. H. A. (1980) Colorimetric and Spectrophotomethc Determination of Hydrasds Alkaloids in Pharmaceutical Preparations. J. Pharm. Sci. 69: 597-598   DOI   PUBMED
7 Akada, Y, Kawano, S. and Tanase, Y. (1980) High-speed liquid chromatographic analysis of drugs. X. Simultaneous determination of aciinol and berbenne chloride in pharma-ceutical preparation. Yakugaku Zasshi 100: 766-770   DOI
8 Namba, T. (1993) The Encyclopedia pf Wakan-Yaku(Tradi-donal Sino-Japanese Medicines) with Color Picture vol. I, P. 154-158. Hoikusha, Osaka
9 Sakai, T. (1983) Spectrophotometnc Determinadon of Trace Amounts of Quatemary Ammonium Salts in Drugs by lon-pair Extracdon with Bromphenol Blue and Quinine. Anatyst 108: 608-614
10 Leonard, I. K. and Elizabeth, G. (1950) Spectrophotometric Studies of Coba1t(II) Thiocyanate Complexes in Organic Solvents. J. Am. Chem. Soc. 72: 5659-5662   DOI
11 PhiliP, W. W. and Charles, G. D. (1951) Nature of the Cobalt Thiocyanate Reacdon. Anal. Chem. 23: 334-337
12 Lin, S. J., Tseng, H. H., Wen, K. C. and Suen, T. T. (1996) Determination of gentiopicroside, mangifeiin, palmatine, berberine, baicalin, wogonin and glycyrrhizin in the traditio-nal Chinese medicinal preparation Sann-Joong-Kuey-Jian-Tang by high-performance liquid chromatography. J. Chromatography A 730: 17-23   DOI   ScienceOn
13 Lee, H. S., Eom, Y. E. and Eom, D. O. (1999) Narrowbore high performance liquid chromatography of berberine and palmatine in crude drugs and pharmaceuticals with ion-pair extraction using cobalt thiocyanate reagent. J. Pharm. Biomed. Anal. 21: 59-63   DOI   ScienceOn