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Quantitative Analysis of Eleutherosides B and E Using HPLC-ESI/MS  

Choi, Young-Hae (College of Pharmacy, Seoul National University)
Kim, Jin-Woong (College of Pharmacy, Seoul National University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.2, 2002 , pp. 88-91 More about this Journal
Abstract
Liquid-chromatography coupled with electrospray-ion trap mass spectrometry was applied to the analysis of the eleutherosides B and E in the Eleutherococcus senticosus cortexes. The optimum ESI/MS results were obtained in the positive ion mode using extracted ion chromatogram targeting Na-adduct molecular ion of each compound. This method allowed rapid and simple gradient separation of underivatized eleutherosides B and E without pre-purification steps at very low concentration.
Keywords
HPLC-ESI/MS; Eleutheroside B; Eleutheroside E; Eleutherococcus senticosus;
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