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Inhibitory Effects of Butyl Alcohol Extract from Caesalpinia sappan L. on Melanogenesis in Melan-a Cells  

Hwang, Sang-Gu (Department of Chonbuk Pathology, College of Oriental Medicine, Wonkwang University)
Lee, Jin-Seon (Amore-Pacific R&D Center)
Baek, Seung-Hwa (Professional Graduate School of Oriental Medicine, Wonkwang University)
Jeon, Byung-Hun (Department of Chonbuk Pathology, College of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
Chun, Hyun-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.2, 2002 , pp. 130-136 More about this Journal
Abstract
Melanogenesis is a physiological process resulting in the synthesis of melanin pigments, which play a crucial protective role against skin photocarcinogenesis. The heart wood of Caesalpinia sappan L.(C. sappan) has long been commonly used in Oriental folk medicines to promote blood circulation, and as an emmenagogue, analgesic or anti-inflammatory agent as well as a remedy for thrombosis. From the heartwood, many constituents have been purified and among them, brazilin and hematoxylin are two of the most abundant. This present study was designed to investigate the inhibitory effect of butanol extract from C. sappan on proliferation and melanogenesis in Melan-a cells. After 48 h treatment of these cells with various concentrations of butanol extract, the cells showed a dose-dependent inhibition in their proliferation without apoptotic cell death. Therefore, the growth retardation by the extract may be due to the cell arrest or cell differentiation. We also estimated total melanin content as a final product and activity of tyrosinase, a key enzyme, of melanogenesis in Melan-a cells. The melanin content and tyrosinase activity were deσeased in extract-treated cells in a dose dependent manner compared to control group. The butanol extract also resulted in a decrease of melanin content in ${\alpha}-melanocyte-stimulating$ hormone (MSH)-induced melanogenesis, indicating that butanol extract of C. sappan could be developed as skin whitening components of cosmetics.
Keywords
Caesalpinia sappan L.; Melanogenesis; Melan-a; Tyrosinase activity; Melanin content;
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1 Kameyama, K., Takemura, T., Hamada, Y., Sakai, C.,Kondoh, S. and Nishi- yama, S. (1993) Pigment production in murine melanoma cells is regulated by tyrosinase,tyrosianse-related protein 1(TRP 1), dopachrome tautomerase(TRP 2) and a melanogenic inhibitor. J. Invest. Dermatol. 100: 126-132   DOI   ScienceOn
2 김창민 외(1997)중약대사전, 청담출판사 P.2420-2423
3 Park, K.J., Kim, E.H., Eun, Y.A., Kang, B.J. and Sung, H.J. (1997) Cytotoxic effect of Korean traditional prescription on the human gastric cancer cell lines. Kor. J. Pharmacogn. 28(4): 233-238
4 Yang M. J., Kim M. G., Lim S., Ann H. S. and Ahn R. M. (1999) Inhibitory effects of water-acetone extracts ofchestnut inner shell, Pine needle and hop on the melanin biosynthesis. J. Pharm. Soc. Kor. 43(4): 494-498
5 Chun, H. J., Choi, E. Y., Yoon, S. C., Nam, H. W. and Woo, W. H. (2001) Inhibitory effects of Atractylodies Rhizoma alba on melanin biosynthesis. J. Pharm. Soc. Kor. 45(3): 269-115
6 Hearing V. J. (1999) Biochemical control of melanogenesis and melanosomal organizadon. Soc. Invest. Dennatol. 4(1): 24-28
7 Hadley, M. E.(1988) Source synthesis, chemistry, secretion and metabolism in the melanotropic peptides, Vol 1, CRC press Boca, Raton FL
8 Chun H. J., Hwang S. G., Kim C. K., Jeon B. H., Baek S. H and Woo W. H. (2002) In vitro Modulation of proliferation and melanization of B16/F10 melanoma cells by Quercetin .J. Pharm. Soc. Kor. 46(1): 75-80
9 Kaufman, R.J. (1991) Vectors used for expression in mammalian cells. Meth. In. Enzymol. 205: 87-92
10 Lim, D.K., Choi, L.U. and Shin, D.h. (1996) Andoxidative acdvity of some solvent extract from Caesalpinia sappan L. Kor. J. Food Sci. Technot. 28(1): 77-82
11 Yaar, M. and Gilchrest B.A. (1991) Human melanocyte growth and differentiation: A decade of new data. J. Invest. Dermatol. 97: 611-615   DOI   ScienceOn
12 Kim, S.G., Kim, Y.M., Khil, L.Y., Jeon, S.D., So, D.S., Moon, C.H. and Moon, C.K.(1998) Brazilin inhibits acdvities of protein kinase C and insulin receptor serine kinase in rat liver. Arch. Pharm. Res. 21(2): 140-146   DOI
13 정진섭.신민교(1990): 도해 생약대사전, P.666-667
14 Kim, Y.S., Noh, Y.K., Lee, G.I., Kim, Y.K., Lee, K.S. and Min, K.R. (1995) Inhibitory effects of herbal medicines on hyalironidase activity. Kor. J. Pharmacogn. 26(3): 265-272
15 Weixiong, L. and Helene, Z.H. (1997) Induced melanin reduces mutations and cell killing in mouse melanoma. Phytochem. Phytobiol. 65: 480-484   DOI   ScienceOn
16 Moon, C.K., Chung, J.H., Lee, Y.M., Lee, S.H., Hwang, G.S., Park, K.S., Mock, M.S., Kim, S.G., Ahn, Y.S. and Ann, J.H. (1988) EIfects of Brazilin on erythrocyte defonnability and its regulated biochemical factors in streptozotocin induced diabedc rats, Arch. Pharm. Res. 11(2): 149-154   DOI   ScienceOn
17 Matinez-Esparza M. (1998) Mechanisms and melanogenesis inhibition by tumor necrosis factor-a in B16/F10 mouse melanoma cells. Eur. .J. Biochem. 225: 139-146
18 Mosmann T. (1983) Rapid colohmetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay. J. Immun. Methods 65: 55-63   DOI   ScienceOn
19 Jeon, W.K., Park, K.J., Kim, S.Y., Ma, J.Y. and Sung, H.J. (1999) In vitro studies on the anticancer effect and topoisomerase I inhibition activity of Caesalpinia sappan L. extract. Kor. J. Pharmacogn. 30(1): 1-6
20 Moon, C.K., Park, K.S., Kim, S.G., Won, H.S. and Chung, J.H. (1992) Brazilin protects cultured rat hepatocytes from BrCCl\{-3}-induced toxicity. Drug Chem. Toxicol. 15(1): 81-91   DOI   ScienceOn
21 Chun H. J., Mun Y. J., Kim J. H., Kim I. K., Jeon B. H. and Woo W. H. (2000) Effect of the aqueous extract of Epimedium koreanum Nakai on melanin formation in B16 mouse melanoma cell line. J. Pharm. Soc. Kor. 44(5): 455-462
22 Jimbow, K., Quevedo, W.C., Fitzpathc, T.B., et al.(1993) Biology of melanocyte. In: Fitzpathc, T.B., Eisen, A.Z., Wolff, K., et al. ed Dermatology in general medicine, 4th ed. New York :McGraw-Hill Book, 261-289
23 Hosoi J., Abe E., Suda T. and Kuroki T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 a-25-dihydroxyvitamin D3 and rednoic acid. Cancer Res. 45: 1474-1478
24 Bell, A. A. and Weeler, M. H. (1986) Biosynthesis and function of fungal melanin. Ann. Rev. Phytophathol. 24: 411-451   DOI   ScienceOn
25 Hwang, G.S., Kim, J.Y, Chang, T.S., Jeon, S.D., So, D.S. and Moon, C.K. (1998) Effects of Brazilin on the phospholipase A2 activity and changes of intracellular free calcium concentration in rat platelets. Arch. Pharm. Res. 21(6): 774-778   DOI   PUBMED   ScienceOn
26 이 강대, 김 정 하(1997) Brazilin as a new sunless tanningagent, 대한화장품학회지, 23(3): 82-85