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Effect of Leweifang on HeLa Cell Vacuolation Induced by Helicobacter.pylori cytotoxin  

Kwon, Dong-Yeul (Department of Internal Medicine, Shanghai University of Traditional Chinese Medicine)
Cai, Gan (Department of Internal Medicine, Shanghai University of Traditional Chinese Medicine)
Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.1, 2002 , pp. 13-17 More about this Journal
Abstract
Helicobacter pylori infection is associated with type B gastritis, peptic ulcer, and gastric cancer. The vacuolation of cells induced by H. pylori is thought to be essential for the initiation and maintenance of gastric infection. The roles of H. pylori cytotoxin, urease, and ammonia in the vacuolation of HeLa cells were determined. Ammonium chloride augmented the neutral red uptake induced by H. pylori toxin. Acetohydroxamic acid (AHA) failed to block the neutral red uptake induced by H. pylori toxin. Leweifang significantly prevented the vacuolation of HeLa cells induced by H. pylori toxin or H. pylori toxin and ammonium chloride. Further investigation is required to determine the mechanisms of Leweifang for the inhibition of vacuole formation of eukaryotic cells in response to the H. pylori toxin.
Keywords
Helicobacter pylori; vacuolation; urease; ammonia; Leweifang;
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