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http://dx.doi.org/10.7473/EC.2021.56.4.254

Synthesis and Antimicrobial Properties of the Chitosan Derivatives  

Lee, Eun Kyoung (Department of Pharmaceutical Biomedical Engineering, Cheongju University)
Kim, You Kyoung (Department of Applied Chemistry, Cheongju University)
Publication Information
Elastomers and Composites / v.56, no.4, 2021 , pp. 254-263 More about this Journal
Abstract
In this study, chitosan obtained after varying extents of deacetylation (i.e., 10%, 30%, and 47%) was employed to introduce antibacterial properties to chitin. The deacetylation reaction completion, wherein the amino group content of chitin was reduced, was ascertained from the FT-IR and NMR analyses. The 47%-deacetylated chitosan exhibited superior antibacterial properties against Bacillus in a disk diffusion test. To further improve these properties, chitosan derivatives were grafted by acrylic acid and acrylamide. The varying concentrations of carboxyl groups, primary amines, and -CH2-CH2- with increasing acrylic acid and acrylamide contents were determined by FT-IR and NMR analyses. The enhanced antibacterial properties of the chitosan derivatives, owing to the increased acrylic acid and acrylamide contents, were revealed by the disk diffusion test. In particular, the derivatives with 1.3% acrylic acid and acrylamide showed the highest antibacterial activity, the bacterial reduction rate against Staphylococcus aureus and Escherichia coli being 99.9%, as observed through the ASTM E2149 standard test.
Keywords
chitosan; chitosan derivatives; antibacterial materials; water-soluble; antimicrobial properties;
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