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http://dx.doi.org/10.9724/kfcs.2015.31.2.112

Determination of Ethyl Carbamate in Maesil Extract and Estimated Daily Intake  

Choi, Bogyoung (Department of Food and Nutrition, Seoul Women's University)
Koh, Eunmi (Department of Food and Nutrition, Seoul Women's University)
Publication Information
Korean journal of food and cookery science / v.31, no.2, 2015 , pp. 112-117 More about this Journal
Abstract
The analytical method for ethyl carbamate (EC) in maesil (Prunus mume) extract was developed with deuterium-labeled ethyl carbamate as an internal standard. Samples were neutralized with an addition of 1 N sodium hydroxide solution, followed by a solid phase extraction with a Chem Elut cartridge. A standard curve exhibited a good linearity with correlation coefficient of 0.9991. The limit of detection (LOD) and limit of quantification (LOQ) were 2.91 ng/g and 8.83 ng/g, respectively. The recovery rate of EC ranged from 91.40% to 120.90%. The precision never exceeded 12.57% (intra-day) and 11.03% (inter-day). Samples were comprised of 24 home-made and 7 commercially-available maesil extracts. Eight home-made samples contained EC at levels between 3.39 and 75.76 ng/g. Three commercially-available samples had EC at levels between 11.67 and 20.16 ng/g. Average daily intakes of EC from maesil extracts for consumers were 0.23 g/kg of body weight. Based on a benchmark dose confidence limit ($BMDL_{10}$) of 0.25 mg/kg of body weight/day, the margin of exposure (MOE) of EC in maesil extract for consumers was 94,150, which is not of concern. Considering that a daily intake of maesil extract has been increasing, further studies on the formation of EC in maesil extract is needed.
Keywords
maesil extract; ethyl carbamate; LOD; LOQ; precision;
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