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http://dx.doi.org/10.9724/kfcs.2011.27.3.001

Antioxidant Activity and Cytotoxic Effect of an Ethanol Extract from Seoritae  

Jeon, Yeon-Hee (Department of Food Science and Nutrition, Kyungpook National University)
Won, Ji-Hye (Department of Food Science and Nutrition, Kyungpook National University)
Kwon, Ji-Eun (Department of Food Science and Nutrition, Kyungpook National University)
Kim, Mee-Ra (Department of Food Science and Nutrition. Center for Beautiful Aging Kyungpook National University)
Publication Information
Korean journal of food and cookery science / v.27, no.3, 2011 , pp. 1-10 More about this Journal
Abstract
The antioxidant activity and cytotoxic effect of an ethanol extract from Seoritae were analyzed to develop new functional food materials. The antioxidant activity of Seoritae was determined by measuring electron donating ability with 1,1-diphenyl-2picrylhydrazyl (DPPH) and 2-2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, as well as the ferric reducing antioxidant power (FRAP) assay. The cytotoxic effect of the Seoritae ethanol extract was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheltetrazolium (MTT) and sulforhodamine B (SRB) assays. As a result, the electron donating abilities of Seoritae against the DPPH and ABTS radicals were 63.75% and 87.68% at 500 ${\mu}g$/assay, respectively. The $IC_{50}$ values of Seoritae in the DPPH and ABTS assays were 385.39 ${\mu}g$/assay (128.46 ${\mu}g/mL$) and 209.39 ${\mu}g$/assay (51.83 ${\mu}g/mL$). Additionally, the FRAP value of Seoritae was 0.84 $FeSO_4$ eq. mM at 800 ${\mu}g$/assay. The total amounts of polyphenols and flavonoids, which indicate the antioxidant capability of Seoritae extract were 1.65 mg/g and 0.59 mg/g, respectively. Moreover, Seoritae extract showed a high cytotoxic effect of up to 81% against human cancer cells, particularly A-549 and HeLa cells. The growth inhibition rate of Seoritae extract against A-549 and HeLa cells was up to 76.48% and 75.67% in the MTT assay, and 78.98% and 80.54% in the SRB assay, respectively. The results of this study suggest that an ethanol extract of Seoritae is a potentially good natural antioxidant.
Keywords
antioxidant; cytotoxicity; phytochemical; Seoritae;
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