Browse > Article
http://dx.doi.org/10.15230/SCSK.2022.48.4.385

Study on Effect of Human Hair by Deer Antler Fermented Keratin Peptide  

Gaewon, Nam (Department of Bio-cosmetic Science, Seowon University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.48, no.4, 2022 , pp. 385-392 More about this Journal
Abstract
In this study, keratin peptides were produced through high-temperature anaerobic fermentation of keratin, a protein contained in deer antler, with Fervidobacterium islandicum AW-1, and factors related to human hair, confirming the possibility of keratin peptides as cosmetic ingredients. As a result of the cytotoxicity and proliferation of deer antler fermented keratin peptide according to the concentration in the dermal papilla cell line, cytotoxicity was not observed and the cell proliferation effect was shown. For human dermal papilla cells, statistically significant increasing in growth factors according to the deer antler fermented keratin peptide was determined possiblity of effects on hair growth. Cosmetic products containing deer antler fermented keratin peptides were manufactured and skin safety and anti hair loss efficacy clinical tests were conducted. As a result, after 12 weeks of use, the total number of hairs statistically significant increased compared to before using the product and the difference in total number of hairs compared to the control group was found. In conclusion, we suggest that the possibility of fermented deer antler keratin peptide as a cosmeceutical ingredient as well as a health functional food material was confirmed.
Keywords
fervidobacterium islandicum aw-1; deer antler; keratin peptide; hair; dermal papillar cell;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 N. J. Gang, H. S. Jin, S. E. Lee, H. J. Kim, and H. Koh, New approaches towards the discovery and evaluation of bioactive peptides from natural resources, Crit Rev Environ Sci Technol., 50(1), 72 (2019).
2 T. Uhlig, T. Kyprianou, F. G. Matinelli, C. A. Oppici, D. Heiligers, D. Hills, X. R. Calvo, and P. Verhaert, The emergence of peptides in the phamaceutical business: From exploration to exploitation, EuPA Open Proteom. 4, 58 (2014).
3 H. Korhonen and A. Pihlanto, Food-derived Bioactive Peptides-Opportunities for Designing Future Foods. Curr Pharm Des, 9(16), 1297 (2003).
4 G. Tucker. B. DeSilva, J. Dressman, M. Ito, T. Kumamoto, D. Mager, H. Mahler, A. H. M. Zeem G. M. Pauletti, H. Sasaki, V. Shah, D. Tang, and M. Ward, Current Challenges and Potential Opportunities for the Pharmaceutical Sciences to Make Global Impact: An FIP Perspective. J. Pharm. Sce. 105(9), 2489 (2016).
5 B. Wang, W. Yang, J. McKittrick, and M. A. Mey ers, Keratin: Structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration, Prog Mater Sci.. 76, 229 (2016).
6 J. Henry , E. Toulza, C. Y. Hsu, L. Pellerin, S. Balica, J. Mazereeuw-Hautier, C. Paul, G. Serre., N. Jonca, and M. Simon, Update on the epidermal differentiation complex. Front Biosci (Landmark Ed), 17(4), 1517 (2012).
7 Y. J. Lee, H. Jeong, G. S. Park, Y. Kwak, S. J. Lee, M. K. Park, J. Y. Kim, H. K. Kang, J. H. Shin, and D. W. Lee, Genome sequence of a native-feather degrading extremely thermophilic Eubacterium, Fervidobacterium islandicum AW-1. Stand Genomic Sci., 29(10), 71 (2015).
8 H. S. Jin, K. Song, J. H. Baek, J. E. Lee, D. J. Kim, G. W. Nam , N. J. Kang, and D. W. Lee, Identification of matrix metalloproteinase-1-suppressive peptides in feather keratin hydrolysate, J. Agric. Food Chem., 66(48), 12719 (2018).
9 G. W. Nam, D. W. Lee, H. S. Lee, N. J. Lee, B. C. Kim, E. A. Choe, J. K. Hwang, M. T. Suhartono, and Y. R. Pyun, Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe, Arch. Microbiol., 178(6), 538 (2002).
10 H. S. Jin, S. Y. Park, K. Kim, Y. J. Lee, G. W. Nam, N. J. Kang, and D. W. Lee, Development of a keratinase activity assay using recombinant chicken feather keratin substrates, PLoS ONE, 12(2), e0172712 (2017).
11 Y. J. Lee, I. Dganasingh, J. S. Ahn, H. S. Jin, J. M. Choi, S. H. Lee, and D. W. Lee, Biochemical and structural characterization of a keratin-degrading M32 carboxypeptidase from Fervidobacterium islandicum AW-1, Biochem. Biophys. Res. Commun., 468, 927 (2015).
12 H. S Jin, S. Y. Park, K. Kim, Y. J. Lee, G. W. Nam, N. J. Kang, and D. W. Lee, Development of a keratinase activity assay using recombinant chicken feather keratin substrates, PLoS ONE. 12(2), e0172712 (2017).
13 S. Kim, S. N. Kim, G. Jeong, M. J. Hong, Y. Lee, S. H. Shin, H. Park, Y. C. Jung, E. J. Kim, B. C. Park, and H. J. Kim, Efficacy of caffeine in promoting hair growth by enhancing intracellular activity of hair follicles, Korea J Cosmetic Science, 1(1), 11 (2019).
14 M. H. Kwack, S. H. Shin, S. R. Kim, S. U. Im, I. S. Han, M. K. Kim, J. C. Kim, and Y. K. Sung, L-Ascorbic Acid 2-Phosphate Promotes Elongation of Hair Shafts via the Secretion of Insulin-like Growth Factor-1 from Dermal Papilla Cells through Phosphatidylinositol 3-Kinase. Br J Dermatol, 160(6), 1157 (2009)   DOI
15 J. Kim, J. Y. Shin, Y. H. Choi, M. Jang, Y. J. Nam, S. Y. Lee, J. H. Jeon, M. H. Jin, and S, Lee, Hair Growth Promoting Effect of Hottuynia Cordata Extract in Cultured Human Hair Follicle Dermal Papilla Cells. Biol Pharma Bulletin, 42(10), 1665 (2019)
16 M. Iino, R. Ehama, Y. Nakazawa, T. Iwabuchi, M. Ogo, M. Tajima, and S. Arase, Adenosine Stimulates Fibroblast Growth Factor-7 Gene Expression Via Adenosine A2b Receptor Signaling in Dermal Papilla Cells. J Invest Dermatol, 127(6), 1318 (2007)
17 W. Li, X. Y. Man, C. M. Li, J. Q. Chen, J. Zhou, S. Q. Cai, Z. F. Lu, and M. Zheng, VEGF Induces Proliferation of Human Hair Follicle Dermal Papilla Cells through VEGFR-2-Mediated Activation of ERK. Exp Cell Res, 318(14), 1633 (2012)   DOI
18 F. M. Tsai, L. K. Wang, M. L. Chen, M. C. Lee, Y. Y. Lin, and C. H. Wang, Induction of Cell Proliferation and Cell Death in Human Follicle Dermal Papilla Cells by Zinc Chloride. Indian J Parm Sci, 81(4), 781 (2019).
19 J. H. Oh, K. H. Jeong, J. E. Kim, and H. Kang, Synthesized Ceramide Induces Growth of Dermal Papilla Cells with Potential Contribution to Hair Growth, Annal Dermatol, 31(2), 164 (2019).
20 S. Y. Ahn, L. Q. Pi, S. T. Hwang, and W. S. Lee, Effect of IGF-I on Hair Growth Is Related to the Anti-Apoptotic Effect of IGF-I and Up-Regulation of PDGF-A and PDGF-B. Annal Dermatol, 24(1), 26 (2012)   DOI
21 S. Lachgar, M. Charveron, Y. Gall, and J. L. Bonafe, Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells. Br J Dermatol, 138, 407 (1998)   DOI