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http://dx.doi.org/10.15230/SCSK.2021.47.4.297

Development of an In Vitro Pigmented Skin Model to Evaluate the Effectiveness of Whitening Functional Cosmetic Ingredients  

Kim, Seolyeong (R&D Institute, Biosolution co., Ltd.)
Lee, Geonhee (R&D Institute, Biosolution co., Ltd.)
Gwak, Eun Ji (R&D Institute, Biosolution co., Ltd.)
Kim, Su Ji (R&D Institute, Biosolution co., Ltd.)
Lee, Su Hyon (R&D Institute, Biosolution co., Ltd.)
Lim, Kyung-Min (College of Pharmacology, Ewha Womans University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.47, no.4, 2021 , pp. 297-304 More about this Journal
Abstract
In this study, we prepared a pigmented skin model, KeraSkin-MTM for the in vitro evaluation of whitening agents. For the purpose of complementing the existing mono-layer cell culture testing method, KeraSkin-MTM was produced through the co-culture of human skin-derived keratinocytes and melanocytes. The efficacy of four well-known whitening agents (arbutin, ascorbic acid, kojic acid, niacinamide) was evaluated in KeraSkin-MTM in order to assess its usefulness in assessing whitening efficacy. As a result, it was possible to observe additional details such as the distribution of melanin granules and melanin capping in each skin layer through KeraSkin-MTM, which was previously difficult to assess in the traditional 2D cell culture system. In addition, quantification through image analysis of KeraSkin-MTM allowed for a statistical analysis of the whitening effects. These results suggest that the KeraSkin-MTM can be used as a new evaluation method of evaluating whitening efficacy, as well as complement the traditional total melanin content and tyrosinase inhibition assays.
Keywords
whitening; alternative test method; pigmented human skin model; $KeraSkin-M^{TM}$;
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1 J. Y. Kim, M. K. Lee, B. S. Hwang, G. C. Kim and I. G. Hwang, Antioxidant activity and tyrosinase inhibitory activities of Codonopsis lanceolata extract and solvent fraction, Korean J. Food Nutr., 32(6), 611 (2019).
2 V. Rogiers, E. Benfenati, U. Bernauer, L. Bodin, P. Carmichael, Q. Chaudhry, P. J. Coenraads, M. T. D. Cronin, M. Dent, M. Dusinska, C. Ellison, J. Ezendam, E. Gaffet, C. L. Galli, C. Goebel, B. Granum, H. M. Hollnagel, P. S. Kern, K. Kosemund-Meynen, G. Ouedraogo, E. Panteri, C. Rousselle, M. Stepnik, T. Vanhaecke, N. Goetz and A. Worth. The way forward for assessing the human health safety of cosmetics in the EU - Workshop proceedings, Toxicology, Luxembourg, 436 152421 (2020).
3 S. M. Shin, H. M. Cho, M. H. Kim, T. E. Kim and Y. W. Shin, 2020 Cosmetic industry analysis report. KHIDI, Korea, 1 (2020).
4 P. Aroca, K. Urabe, T. Kobayashi, K. Tsukamoto, and V. J. Hearing, Melanin biosynthesis patterns following hormonal stimulation, J. Biol. Chem., 268(34), 25650(1993).   DOI
5 J. Thingne s, T. J. Lavelle , E. Hovig and S. W. Omholt, Understanding the melanocyte distribution in human epidermis: An agent-based computational model approach, PoLS One. 7(7), e40377 (2012).   DOI
6 Y. U. Jeong, H. Lee, H. N. Park, K. M. Kim, S. Y. Kim, and Y. J. Park, Studies on antioxidant, anti-inflammation and tyrosinase inhibitory activities of Melissa officinalis extracts and their fractions, J. Soc. Cosmet. Sci. Korea, 44(4), 465 (2018).   DOI
7 J. M. Gillbro and M. J. Olsson, The me lanoge ne sis and mechanisms of skin-lightening agents - existing and new approaches, Int. J. Cosmet. Sci., 33(3), 210 (2011).   DOI
8 E. J. Seo, E. S. Hong, M. H. Choi, K. S. Kim and S. J. Lee, The antioxidant and skin whitening effect of Artemisia iwayomogi extracts, Korean J. Food Sci. Technol., 44(1), 89 (2012).   DOI
9 H. J. Kim, H. R. Choi, D. S. Kim and K. C. Park, Topical hypopigmenting agents for pigmentary disorders and their mechanisms of action, Ann. Dermatol., 24(1), 1 (2012).   DOI
10 B. A. Kim, Inhibitory effects of fractions from Glycine soja Siebold et Zucc. on melanogenesis in B16F10 melanoma cells, J. Soc. Cosmet. Sci. Korea, 43(3), 231 (2017).   DOI
11 W. Russel and R. Burch, The principles of humane experimental technique, 238, Methuen, London UK (1959).
12 P. Flecknell, Replacement, reduction and refinement, ALTEX. 19(2), 73 (2002).
13 Y. S. Song, B. Y. Chung, W. G. Cho and S. H. Kang, Development of polyethoxylated ascorbic acid as a whitening agent, J. Soc. Cosmet. Sci. Korea, 26(1), 199 (2000).
14 S. W. Jung, N. K. Lee, S. J. kim and D. S. Han, Screening of tyrosinase inhibitor from plants, Korean J. Food Sci. Technol., 27(6), 891 (1995).
15 W. Qian, W. Liu, D. Zhu, Y. Cao, A. Tang, G. Gong and H. Su, Natural skinwhitening compounds for the treatment of melanogenesis (review), Exp. Ther. Med., 20(1), 173 (2020).   DOI
16 M. G. Choung, Y. S. Hwang, G. P. Kim, K. G. Ahn, H. S. Shim, S. B. Hong, J. H. Choi, C. Y. Yu, I. M. Chung, S. H. Kim and J. D. Lim, Antimelanogenic effect and whitening of anthocyanin rich fraction from seeds of Liriope platyphylla, Korean J. Medicinal Crop Sci. 21(5), 361 (2013).   DOI