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http://dx.doi.org/10.15230/SCSK.2021.47.1.65

Development of a Cosmetic Ingredient Containing DHA Derivatives for Anti-inflammation, Anti-wrinkle, and Improvement of Skin Barrier Function  

Lee, Miyoung (Healthcare Technology Institute, Kolon Advanced Research Center)
Lee, Gil-Yong (Healthcare Technology Institute, Kolon Advanced Research Center)
Suh, Jinyoung (Healthcare Technology Institute, Kolon Advanced Research Center)
Lee, Kyung min (Healthcare Technology Institute, Kolon Advanced Research Center)
Lee, Woojung (Healthcare Technology Institute, Kolon Advanced Research Center)
Cho, Hee Won (Healthcare Technology Institute, Kolon Advanced Research Center)
Yi, Jong-Jae (Microbial Biotechnology Research Center)
Seo, Jeong-Woo (Microbial Biotechnology Research Center)
Choi, Heonsik (Healthcare Technology Institute, Kolon Advanced Research Center)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.47, no.1, 2021 , pp. 65-73 More about this Journal
Abstract
It is very important to control the inflammation of the skin because it can develop into diseases such as atopy as well as scarring and aging. In this work, we recently identified the in vitro synthesis of specialized pro-resolving mediators (SPMs) known to control inflammation in the human body and the applicability of cosmetics. Using recombinant protein of lipoxygenase from Glycine max, we succeeded to prepare mixtures of mono- or di-hydroxy DHA named as S-SPMs and used them for in vitro efficacy test. To investigate anti-inflammatory effect of S-SPMs, mRNA level of TNF-α and IL-6 were analyzed. Under UVB exposed condition, expression of both were decreased by S-SPMs treatment. And we observed reduced production of nitric oxide (NO) by S-SPMs application under the condition with diesel particulate matter (DPM). At the same experimental condition, malondialdehyde (MDA) production was decreased by S-SPMs, indicating the inhibitory effect of S-SPMs in lipid peroxidation. In addition, S-SPMs treatment resulted in reduction of matrix metalloproteinases-1 (MMP-1) expression and elevation of procollagen type I synthesis. Together with this, mRNA level of filaggrin and loricrin were increased by S-SPMs, indicating enhancement of skin barrier function. These results demonstrate that S-SPMs is a good candidate to develop as a cosmetic ingredient for anti-inflammation, anti-wrinkle, and improvement of skin barrier function.
Keywords
specialized pro-resolving mediators; lipoxygenase; anti-inflammation; anti-wrinkle; skin barrier;
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1 Q. Qu, W. Xuan, and G. H. Fan, Roles of resolvins in the resolution of acute inflammation, Cell Biol. Int., 39(1), 3 (2015).   DOI
2 M. Spite, J. Claria, and C. N. Serhan, Resolvins, specialized pro-resolving lipid mediators, and their potential roles in metabolic diseases, Cell Metab, 19(1), 21 (2014).   DOI
3 Y. Zhuang and J. Lyga, Inflammaging in skin and other tissues - The roles of complement system and macrophage, Inflamm. Allergy Drug Targets, 13(3), 153 (2014).   DOI
4 E. P. Dobson, C. J. Barrow, J. A. Kralovec, and J. L. Adcock, Controlled formation of mono- and dihydroxyresolvins from EPA and DHA using soybean 15-lipoxygenase, J. Lipid Res., 54(5), 1439 (2013).   DOI
5 I. A. Butovich, A one-step method of 10,17-dihydro(pero) xydocosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid synthesis by soybean lipoxygenase, J. Lipid Res., 47(4), 854 (2006).   DOI
6 S. P illai, C. Oresajo, and J. Hayward, Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a revie, Int. J. Cosmet. Sci. 27(1), 17 (2005).   DOI
7 D. Fagot, D, Asselineau, and F. Bernerd, Matrix metalloproteinase-1 production observed after solar-simulated radiation exposure is assumed by dermal fibroblasts but involves a paracrine activation through epidermal keratinocytes, Photochem. Photobiol, 79(6), 499 (2004).   DOI
8 J. N. Sharma, A. Al-Omran, and S. S. Parvathy, Role of nitric oxide in inflammatory diseases, Inflammopharmacology, 15(6), 252 (2007).   DOI
9 P. Pittayapruek, J. Meephansan, O. Prapapan, M. Komine, and M. Ohtsuki, Role of matrix metalloproteinases in photoaging and photocarcinogenesis, Int J Mol Sci, 17(6), 868 (2016).   DOI
10 A. Ayala, M. F. Munoz, and S. Arguelles, Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal, Oxid Med Cell Longev., 2014, 1 (2014).
11 K. M. Pawelec, S. M. Best, and R. E. Cameron, Collagen: a network for regenerative medicine, J Mater Chem B Mater Biol Med, 4(40), 6484 (2016).   DOI
12 T. H. Kim, G. D. Kim, Y. H Jin, Y. S. P ark, and C. S. Park, Omega-3 fatty acid-derived mediator, Resolvin E1, ameliorates 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC /Nga mice, Int. Immunopharmacol., 14(4), 384 (2012).   DOI
13 S. Kezic and I. Jakasa, Filaggrin and skin barrier function, Curr Probl Dermatol, 49, 1 (2016).   DOI
14 A. E. Barden, E. Mas, and T. A Mori, n-3 Fatty acid supplementation and proresolving mediators of inflammation, Curr Opin Lipidol., 27(1), 26 (2016).   DOI
15 L. V Norling, L. Ly, and J. Dalli, Resolving inflammation by using nutrition therapy: Roles for specialized pro-resolving mediators, Curr Opin Clin Nutr Metab Care, 20(2), 145 (2017).   DOI