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http://dx.doi.org/10.15230/SCSK.2021.47.1.57

Analytical Method Development of Isoscoparin in Silene seoulensis Extract Using HPLC  

Kwon, Jin Gwan (Gyeonggido Business and Science Accelerator)
Seo, Changon (Gyeonggido Business and Science Accelerator)
Jung, Yeon Woo (Gyeonggido Business and Science Accelerator)
Choi, Yongmun (Gyeonggido Business and Science Accelerator)
Shin, Hyun Tak (DMZ Botanic Garden, Korea National Arboretum)
Jung, Su Young (DMZ Botanic Garden, Korea National Arboretum)
Choi, Jeong June (College of Korean Medicine, Daejeon University)
Kim, Jin Kyu (Gyeonggido Business and Science Accelerator)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.47, no.1, 2021 , pp. 57-63 More about this Journal
Abstract
In this study, isoscoparin was selected as an indicator component to develop Silene seoulensis extract as a functional cosmetic material, and we developed an analysis method using high performance liquid chromatography (HPLC) for quality control. HPLC was performed on a Unison US-C18 with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methanol at a flow rate of 1.0 mL/min at 35 ℃, and the detection wavelength was 330 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.02 and 0.07 mg/mL respectively. Calibration curves showed good linearity (R2 > 0.99988), and the precision of analysis was satisfied (less than 0.46%). In addition, the recovery rate was in the range of 99.10 to 101.61%, it was shown to be accurate. This result indicated that the established HPLC method is very useful for the determination of marker compounds in Silene seoulensis extracts.
Keywords
silene seoulensis; isoscoparin; functional cosmetic; HPLC; validation;
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