Browse > Article
http://dx.doi.org/10.15230/SCSK.2018.44.4.419

Analysis of Procollagen Biosynthesis of Functional Peptides Utilizing Stiffness Controlled Artificial Skin Dermis  

Byun, Jina (Dept. of Chemical Engineering, Soongsil University)
Shin, Sung Gyu (Dept. of Chemical Engineering, Soongsil University)
Han, Sa Ra (Dept. of Chemical Engineering, Soongsil University)
Cho, Sung Woo (Dept. of Chemical Engineering, Soongsil University)
Lim, Jun Woo (Dept. of Chemical Engineering, Soongsil University)
Jeong, Jae Hyun (Dept. of Chemical Engineering, Soongsil University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.44, no.4, 2018 , pp. 419-425 More about this Journal
Abstract
In this study, cross-linked collagen gels were successfully prepared with varying of elastic modulus from 0.7 to 17.7 kPa using a chemical cross-linker. Then, human dermal fibroblasts were encapsulated into the porous pores introduced into the gels, and cell growth and behavior were examined by gel's mechanical properties. Specifically, increasing elastic modulus of the gel led to decreases in procollagen synthesis from 47 to 32 ng. In addition, there could be optimum elastic modulus for procollagen production, when the gels were treated with adenosine. However, interestingly, this study discovered that the procollagen production level was not influenced by the elastic modulus of the gel for functional peptide. In conclusion, these results would be highly useful for designing reconstructed skins with varying of elastic modulus to examine functional materials in cosmetics.
Keywords
collagen gel; glutaraldehyde; elastic modulus; functional peptide; pro-collagen;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Y. Ahn, C. Y. Lee, S. Baek, T. Kim, P. Kim, S. Lee, D. Min, H. Lee, J. Kim, and W. G. Jung, Quantitative monitoring of laser-treated engineering skin using optical coherence tomography, Biomed. Opt. Express., 7(3), 1030 (2016).   DOI
2 E. Ruoslahti and M. D. Pierschbacher, New perspectives in cell adhesion: RGD and integrins, Science, 238, 491 (1987).   DOI
3 M. Jung, S. G. Shin, J. W. Lim, S. R. Han, H. Kim, and J. H. Jeong, Tuning the stiffness of dermal fibroblast-encapsulating collagen gel by sequential cross-linking, J. Soc. Cosmet. Sci. Korea, 44(1), 23 (2018).   DOI
4 M. W. Tibbitt and K. S. Anseth, Hydrogels as extracellular matrix mimics for 3D cell culture, Biotechnol. Bioeng., 103, 665 (2009).
5 E. Proksch, M. Schunck, V. Zague, D. Segger, J. Degwert, and S. Oesser, Oral intake of specific bioactive collagen peptides reduces skin wrinkles and increases dermal matrix synthesis, Skin Pharmacol. Physiol., 27(3), 113 (2014).   DOI
6 J. M. Ruijgrok, J. R. Wijn, and M. E. Boon, Glutaraldehyde crosslinking of collagen: effects of time, temperature, concentration and presoaking as measured by shrinkage temperature, Clin. Mater., 17, 23 (1994).   DOI
7 J. H. Jeong, Y. Liang, M. Jang, C. Cha, C. Chu, H. Lee, W. Jung, J. W. Kim, S. A. Boppart, and H. Kong, Stiffness-modulated water retention and neovascularization of dermal fibroblast-encapsulating collagen gel, J. Tissue Eng. Part A, 19(11), 1275 (2013).   DOI
8 P. Zorlutuna, J. H. Jeong, H. Kong, and R. Bashir, Stereolithography-based hydrogel microenvironments to examine cellular interactions, Adv. Funct. Mater., 21(19), 3642 (2011).   DOI
9 S. J. Oh, H. Kim, S. W. Cho, S. R. Ham, S. G. Shim, B. H. Han, and J. H. Jeong, Tuning the toughness of an alginate micro-gel for cell-instructed delivery, Polym. Kor., 42(1), 140 (2018).   DOI
10 H. Kim, Y. N. Cho, S. W. Cho, Y. Kim, J. H. Jeong, and H. W. Ryu, Tuning the hydrophobicity of agar hydrogel with substituent effect, Polym. Kor., 40(2), 1 (2016).   DOI
11 S. Jose, M. L. Hughbanks, B. Y. Binder, G. C. Ingavle, and J. K. Leach, Enhanced trophic factor secretion by mesenchymal stem/stromal cells with glycine-histidine-lysine (GHK)-modified alginate hydrogels, Acta Biomater., 10(5), 1955 (2014).   DOI
12 T. Kwon, C. T. Oh, E. J. Choi, S. R. Kim, Y. Jang, E. J. Ko, K. H. Yoo, and B. J. Kim, Conditioned medium from human bone marrow-derived mesenchymal stem cells promotes skin moisturization and effacement of wrinkles in UVB-irradiated SKH-1 hairless mice, Photodermatol. Photoimmunol. Photomed., 32(3), 120 (2016).   DOI
13 J. H. Jeong, J. J. Schmidt, C. Cha, and H. J. Kong, Tuning responsiveness and structural integrity of a pH responsive hydrogel using a poly (ethylene glycol) cross-linker, Soft Matter, 6, 3930 (2010).   DOI
14 T. L. Tuan, L. C. Keller, D. Sun, M. E. Nimni, D. Cheung, Dermal fibroblasts activate keratinocyte outgrowth on collagen gels, J. Cell. Sci., 107, 2285 (1994).
15 M. El-Domyati, S. Attia, F. Saleh, D. Brown, D. E. Birk, F. Gasparro, H, Ahmad, and J. Uitto, Intrinsic aging vs. photo-aging: a comparative histopathological, immunohistochemical and ultrastructural study of skin, Exp. Dermatol., 11(5), 398 (2002).   DOI