Browse > Article
http://dx.doi.org/10.15230/SCSK.2017.43.2.87

Cytoprotective Effect of Petasites japonicus Extract on Cadmium-induced Cytotoxicity in HaCaT cell  

Kim, Bo-Ae (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.43, no.2, 2017 , pp. 87-92 More about this Journal
Abstract
In this study, we investigated the effect of Petasites japonicus extract on the cytotoxicity and cytoprotective effects against cadmium for cosmetics use. We measured the protein expression of apoptosis regulatory factor (Bcl-2 and procaspase-3) after treatment of Petasites japonicus extract in the cadmium-induced keratinocyte. As a result, high cell viabilities above 98% were observed in the all treated concentrations except at $200{\mu}g/mL$ of Petasites japonicus extract in keratinocytes with cadmium-induced damages. In keratinocytes with cadmium-induced damages, Bcl-2 and procaspase-3 protein expression increased in the experimental group treated with Petasites japonicus extract. Also HaCaT cells resulted in cleavage of PARP protein at 12 h post-cadmium exposure. Western blot analysis and relative density of the bands suggested that pretreatment of cells with Petasites japonicus extract inhibited cadmium-mediated cleavage of PARP. These results suggest that Petasites japonicus extract can be used as the cosmetic ingredients for cytoprotective effect.
Keywords
Petasites japonicus; cell protection; keratinocyte; cadmium;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 H. H. Kim, Y. W. Lim, J. Y. Yang, K. H. Moon, and D. C. Shin, Distribution of inorganic metals in blood of adults in urban area of Seoul, Korea. J. Environ. Toxicol., 19. 327 (2004).
2 C. Jeon, J. Y. Park, and T. J. Yoo, Characteristics of metal removal using carboxylated alginic acid, Water Res., 36, 1814 (2002).   DOI
3 Y. H. Kim, Y. J. Yoo, and Y. J. Lee, Characterization of lead adsorption by Undaria pinnatifida. Biotechnol Lett., 17, 345 (1995).   DOI
4 S. H. Oh, Y. H. Yang, O. Y. Kwan, and M. R. Kim, Effects of diet with added butterbur (Petasites japonicus Maxim) on the plasma lipid profiles and antioxidant index of mice. J. East Asian Soc. Dietary Life, 16(4), 399 (2006).
5 B. S. Cho, J. J. Lee, J. O. Ha, and M. Y. Lee, Physicochemical composition of Petasites japonicus, Korean J. Food Preserv., 13(5), 661 (2006).
6 Y. Yaoita and M. Kikuchi, Petasiphenone a phenolic compound form rhizomes of Petasites Japonicus. Phytochem., 37, 1773 (1994).   DOI
7 M. Kikuchi, Studies on the constituents of the flower stalk of Petasites japonicus Maxim VII. on the components of the volatile oil. Yakugaku Zasshi, 93, 123 (1973).   DOI
8 O. B. Choi, Anti-allergic effects of Petasites japonicum, Korean J. Food & Nutr., 15(4), 382(2002).
9 Y. H. Jee and C. S. Lee, Pathological changes on rats and mice fed with Petasites japonicus Maxim, Korean J. Vet. Res., 36(2), 417 (1996).
10 C. H. Lee, M. C. Chung, H. J. Lee, and Y. H. Kho, An apoptosis regulator isolated from Petasites japonicus, Korean J. Food Sci. Technol., 32(2), 448 (2000).
11 H. S. Seo, B. H. Chung, and Y. G. Cho, The antioxidant and anticancer effects of butterbur (Petasites japonicus) extracts, Korean J. Plant Res., 21(4), 265 (2008).
12 J. H. Kim, Y. Na, G. S. Sim, B. C. Lee, and H. B. Pyo, Antioxidative and anti-inflammatory effects of Petasites japonicus. J. Soc. Cosmet. Sci. Korea, 32(4), 263 (2006).
13 C. Soldani and A. I. Scovassi, Poly (ADP-ribose) polymerase-1 cleavage during apoptosis: Apoptosis, 7, 321 (2002).   DOI
14 S. H. Seo, G. S. Bae, S. B. Choi, I. J. Jo, D. G. Kim, J. Y. Shin, H. J. Song, S. J. Park, and M. O. Choi, The antioxidative and cytoprotective effect of Lonicerae japonicae FLOS water extracts on the ultraviolet (UV) B-induced human HaCaT keratinocyte, Kor. J. Herbology, 29(6), 63 (2014).   DOI
15 A. Koh, S. Choi, Y. U. Kim, and G. H. Park, Protective effects of Pyrus pyrifolia NAKAI leaf extracts on UVB-induced toxicity in human dermal fibroblast, J. Soc. Cosmet. Sci. Korea, 42(1), 87 (2016).   DOI