Browse > Article
http://dx.doi.org/10.15230/SCSK.2017.43.1.53

Whitening and Anti-inflammatory Constituents from the Extract of Citrullus lanatus Vines  

Jeon, Ah Lim (Department of Chemistry and Cosmetics, Jeju National University)
Kim, Jung Eun (Department of Chemistry and Cosmetics, Jeju National University)
Lee, Nam Ho (Department of Chemistry and Cosmetics, Jeju National University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.43, no.1, 2017 , pp. 53-60 More about this Journal
Abstract
In this study, we investigated whitening and anti-inflammatory constituents from a watermelon (Citrullus lanatus, C. lanatus) vines (leaves and stems). As anti-melanogenesis and anti-inflammatory activities were screened for the ethanol extract and solvent fractions, n-hexane (n-Hex) and ethyl acetate (EtOAc) fractions showed the most potent activities. Three constituents were isolated from the n-Hex and EtOAc fractions of C. lanatus; ${\alpha}-linolenic$ acid (1), sigmast-7-en-O-${\beta}$-D-glucopyranoside (2), 1-feruloyl-${\beta}$-D-glucopyrinoside (3). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including $^1H$ and $^{13}C$ NMR spectra, as well as comparison of the data to the literature values. Whitening and anti-inflammatory effects were studied for the isolated compounds. Upon the anti-melanogenesis tests using ${\alpha}-MSH$ stimulated B16F10 melanoma cells, the compounds 1 and 3 inhibited the cellular melanogenesis and intracellular tyrosinase activities effectively. For the anti-inflammation tests using lipopolysaccharide (LPS)-induced RAW 264.7 cells, the isolates 1 and 3 were determined to decrease the production of nitric oxide (NO) and pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6). Based on these results, C. lanatus vines extract could be potentially applicable as whitening and anti-inflammatory ingredients in cosmetic formulations.
Keywords
Citrullus lanatus; whitening; anti-inflammation; constituents;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 E. J. Kwon, H. J. Park, M. M. Kim, K. R. Lee, I. Hong, D. G. Lee, and Y. H. Oh, Effect of Ulmus macrocapa ethanolic extracts on anti-oxidant activity and melanin synthesis in B16F1 cells, J. Life. Sci., 24(9), 946 (2014).   DOI
2 J. J. Ahn, T. Y. Hwang, and H. S. Kim, Study on the physiological activities of Cleyera japonica extract, Korean J. Plant Res., 28(2), 153 (2015).   DOI
3 S. T. Lee, Y. R. Jeong, M. H. Ha, S. H. Kim, M. W. Byun, and S. K. Jo, Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extract in mouse macrophage, J. Korean Soc. Food Sci. Nutr., 29(2), 342 (2000).
4 T. Ljung, S. Lundberg, M. Varsanyi, C. Ohansson, P. T. Schmidt, and M. Herulf, Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus non-responders, World Gastroenterol., 12(21), 3386 (2006).   DOI
5 J. B. Jeong, S. C. Hong, H. J. Jeong, and J. S. Koo, Anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells, Korean J. Plant Res., 25(3), 299 (2012).   DOI
6 T. J. Guzik, R. Korbut, and T. Adamek-guzik, Nitric oxide and superoxide in inflammation and immune regulation, J. Physiol. Pharmacol., 54(4), 469 (2003).
7 R. G. Kim, K. M. Shin, S. K. Chun, S. Y. Ji, S. H. Seo, H. J. Park, J. W. Choi, and K. T. Lee, In vitro antiinflammatory activity of the essential oil from Ligularia fischeri var. spiciformis in murine macrophage RAW 264.7 cells, Yakhak Hoeji, 46(5), 343 (2002).
8 M. S. Nanes, Tumor necrosis factor-${\alpha}$: molecular and cellular mechanisms in skeletal pathology, Gene, 321, 1 (2003).   DOI
9 A. S. Chauhan, P. S. Negi, and R. S. Ramteke, Antioxidant and antibacterial activities of aqueous extract of seabuckthorn (Hippophae rhamnoides L.) seeds, Fitoterapia, 78(7), 590 (2007).   DOI
10 Y. F. Leung, P. O. Tam, W. S. Lee, D. S. Lam, H. F. Yam, B. J. Fan, C. C. Tham, J. K. Chua, and C. P. Pang, The dual role of dexamethasone on anti-inflammation and outflow resistance demonstrated in cultured human trabecular meshwork cells, Mol. Vis., 9, 425 (2003).
11 D. Rocksen, B. Lilliehook, R. Larsson, T. Johansson, and A. Bucht, Differential anti-inflammatory and anti-oxidative effects of dexamethasone and N-acethylcysteine in endotoxin-induced lung inflammation, Clin. Exp. Immunol., 122(2), 249 (2000).   DOI
12 N. K. Kim, M. H. Kim, C. S. Yoon, and S. W. Choi, Studies on the anti-inflammatory activity of Paulownia coreana Uyeki leaf extract, J. Soc. Cosmet. Scientists Korea, 32(4), 241 (2006).
13 T. Kikuchi, A. Ikedaya, A. Toda, K. Ikushima, T. Yamakawa, R. Okada, T. Yamada, and R. Tanaka, Pyrazole alkaloids from watermelon (Citrullus lanatus) seeds, Phytochem. Lett., 12, 94 (2015).   DOI
14 P. Madhavi, R. Maruthi, V. Kamala, R. Habibur, and M. C. Eswaraiash, Evaluation of anti-inflammatory activity of Citrullus lanatus seed oil by in vivo and in vitro models, Int. Res J Pharm. App Sci., 2(4), 104 (2012).
15 S. I. Abdelwahab, L. E. A. Hassan, H. M. Sirat, S. M. A. Yagi, W. S. Koko, S. Mohan, M. M. E. Taha, S. Ahmad, C. S. Chuen, P. Narrima, M. M. Rais, and A. H. A. Hadi, Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzyme inhibition, Fitoterapia, 82(8), 1190 (2011).   DOI
16 J. S. Kim, Y. S. Kwon, Y. J. Sa, and M. J. Kim, Isolation and identification of sea buckthorn (Hippophae rhamnoides) phenolics with antioxidant activity and ${\alpha}$-glucosidase inhibitory effect, J. Agric. Food Chem., 59(1), 138 (2011).   DOI
17 L. E. A. Hassan, H. M. Sirat, S. M. A. Yagi, W. S. Koko, and S. I. Abdelwahab, In vitro antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides (wild melon), J. Med. Pant. Res., 5(8), 1338 (2011).
18 M. A. Alamsjah, S. Hirao, F. Ishibashi, and Y. M. Fujita, Isolation and structure determination of algicidal compounds, Biosci. Biotechnol. Biochem., 69(11), 2186 (2005).   DOI
19 J. Tang, M. H. Qiu, X. M. Zhang, and L. G. Zhou, Chemical constituents from stem of Cucumis sativus L., Nat. Prod. Res. Dev., 21(1), 66 (2009).