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http://dx.doi.org/10.15230/SCSK.2016.42.2.145

Activating the Proliferation of Keratinocyte Stem Cells by Paeonol, a Compound from Natural Herb  

Kim, Do Hyung (Skin Research Center, Research Park, LG Household & Healthcare Ltd.)
Kim, Hyo Jin (Skin Research Center, Research Park, LG Household & Healthcare Ltd.)
Yeo, Hyerin (Skin Research Center, Research Park, LG Household & Healthcare Ltd.)
Lee, Cheon Goo (Skin Research Center, Research Park, LG Household & Healthcare Ltd.)
Lee, Sang Hwa (Skin Research Center, Research Park, LG Household & Healthcare Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.2, 2016 , pp. 145-152 More about this Journal
Abstract
Epidermis is continuously regenerated by keratinocyte stem cells (KSCs) residing in basement membrane, which is critical to the survival of an organism. KSCs are believed to persist during the whole lifetime and generate an enormous number of keratinocytes, required for the maintenance of epidermis, through transit amplifying cells dividing definite times until they become differentiated. In this report, we have developed a phenolic compound, paeonol, purified from Moutan Cortex, as a KSC proliferation activator, by screening about 350 herbal compounds. The cell proliferation activation by paeonol is specific for KSC not for keratinocyte, and no significant difference in the expression of p63 protein, a KSC marker, in KSCs treated with paeonol was observed in FACS analysis with anti-p63 antibody. In the colony forming assay, paeonol-treated KSC showed improved colony forming activity more than 1.3 fold. In addition, the result of PCR array shows that the activity of paeonol is through several signal pathways involving stem cell functions. These results suggest that paeonol could enhance KSC proliferation activity without reduction in stemness and could be applied to cosmetics as a KSC activating ingredient.
Keywords
anti-aging; paeonol; keratinocyte stem cell; p63 protein; cosmetics;
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