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http://dx.doi.org/10.15230/SCSK.2016.42.1.95

Effect of Hydrosol Extracted from Chrysanthemum boreale Makino Flower on Proliferation and Migration in Human Skin Keratinocyte  

Kim, Do Yoon (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
Hwang, Dae Il (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
Yoon, Mi-so (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
Choi, In Ho (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
Lee, Hwan Myung (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.1, 2016 , pp. 95-101 More about this Journal
Abstract
In the present study, we extracted the hydrosol from flower of Chrysanthemum boreale Makino (CBMF hydrosol) by steam distillation and tested the effect of the CBMF hydrosol on skin regeneration using normal human keratinocytes (HaCaTs). CBMF hydrosol induced proliferation as well as migration in HaCaTs in a dose-dependent manner. Treatment with $1{\mu}g/mL$ CBMF hydrosol increased proliferation to $143.71{\pm}3.37%$ and migration to $139.98{\pm}5.72%$ compared with a control group. CBMF hydrosol also significantly enhanced the phosphorylations of extracellular signal-regulated kinase (Erk) 1/2 and serine/threonine-specific protein kinase (Akt) in HaCaTs. Moreover, CBMF hydrosol dose-dependently induced sprout outgrowth in HaCaTs. These results demonstrate that CBMF hydrosol has skin regeneration and wound healing activity in HaCaTs. Therefore, CBMF hydrosol could be used as a potential cosmetic material.
Keywords
Chrysanthemum boreale Makino; keratinocyte; proliferation; migration; skin regeneration;
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