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http://dx.doi.org/10.15230/SCSK.2016.42.1.57

Inhibitory Effects of Dunaliella salina Extracts on Thermally-Induced Skin Aging  

Joo, Ji-Hye (Greensolutions Co., Ltd. R&D center)
Seok, Ji Hyun (Radiant Co., Ltd. R&D center)
Hong, In-kee (Radiant Co., Ltd. R&D center)
Kim, Nam Kyoung (Greensolutions Co., Ltd. R&D center)
Choi, Eunmi (Greensolutions Co., Ltd. R&D center)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.1, 2016 , pp. 57-64 More about this Journal
Abstract
Just like UV radiation, heat increases collagen degradation and accumulation of abnormal elastin fiber and this is termed thermal skin aging. Dunaliella salina (DS), a green alga, is known for its beta-carotene accumulation, having various applications in the health and nutritional products. However, the effects of DS on heat-induced skin aging remain unexplored. In this study, we performed anti-thermal aging tests of the ethanol extract of DS (DSE). We measured the cellular levels of type I procollagen and MMP-1 using ELISA in human dermal fibroblast cells after heat shock. DSE reduced the expression of MMP-1 protein and increased the expression of type I procollagen. In addition, DSE upregulated the mRNA expression of HSP47 reduced by heat shock, which is involved in collagen synthesis. Also, DSE reduced the expression of inflammation mediator (TGF-${\beta}$, IL-12, etc). We demonstrate that DSE regulates the heat-induced solar elastosis through the regulation of tropoelastin and fibrillin-1, two major proteins of elastic fibers, and MMP-12 expression. These results suggest that DSE may be effective for preventing thermally induced skin aging.
Keywords
Dunaliella salina; thermal aging; heat shock; type I procollagen; solar elastosis;
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1 B. A. Gilchrest, Skin aging and photoaging: an overview, J. Am. Acad. Dermatol., 21(3), 610 (1989).   DOI
2 I. M. Braverman and E. Fonferko, Studies in cutaneous aging: I. the elastic fiber network, J. Invest. Dermatol., 78(5), 434 (1982).   DOI
3 W. Montagna, S. Kirchner, and K. Carlisle, Histology of sun-damaged human skin, J. Am. Acad Dermatol., 21(3), 907 (1989).   DOI
4 S. Cho, M. H. Shin, Y. K. Kim, J. E, Seo, Y. M. Lee, C. H. Park, and J. H. Chung, Effects of infrared radiation and heat on human skin aging in vivo, J. Invest. Derm. Symp P., 14(1), 15 (2009).   DOI
5 S. Cho, M. J. Lee, M. S. Kim, S. Lee, Y. K. Kim, D. H. Lee, C. W. Lee, K. H. Cho, and J. H. Chung, Infrared plus visible light and heat from natural sunlight participate in the expression of MMPs and type I procollagen as well as infiltration of inflammatory cell in human skin in vivo, J. Dermatol. Sci., 50(2), 123 (2008).   DOI
6 J. H. Seo and J. H. Chung, Thermal aging: a new concept of skin aging, J. Dermatol. Sci., 2, S13 (2006).
7 C. H. Park, M. J. Lee, J. Ahn, S. Kim, H. H. Kim, K. H. Kim, H. C. Eun, and J. H. Chung, Heat shock-induced matrix metalloproteinase (MMP)-1 and MMP-3 are mediated through ERK and JNK activation and via an autocrine interleukin-6 loop, J. Invest. Dermatol., 123, 1012 (2004).   DOI
8 P. Brenneisen, R. Blaudschun, M. Bernards, J. Wenk, M. Wlaschek, W. Meyer-Ingold, M. Schauen, T. Krieg, and K. Scharffetter-Kochanek, Increased temperature: a potentially important side-effect of ultraviolet radiation treatment leading to induction of interstitial collagenese/matrix metalloproteinase-1, Br. J. Dermatol., 139(3), 537 (1998).   DOI
9 E. G. Hitraya, J. Varga and S. A. Jimenez, Heat shock of human synovial and dermal fibroblasts induces delayed up-regulation of collagenease-gene expression, Biochem. J., 308(3), 743 (1995).   DOI
10 B. A. Vance, C. G. Kowalski, and C. E. Brinckerhoff, Heat shock of rabbit synovial fibroblasts increases expression of mRNAs for two metalloproteinases, collagenase and stromelysin, J. Cell. Biol., 108, 2037 (1989).   DOI
11 M. H. Shin, Y. J. Moon, J. E. Seo, Y. Lee, K. H. Kim, and J. H. Chung, Reactive oxygen species produced by NADPH oxidase, xanthine oxidase and mitochondiral electron transport system mediate heat shock-induced MMP-1 and MMP-9 expression, Free. Radic. Biol. Med., 44(4), 635 (2008).   DOI
12 Z. Chen, J. Y. Seo, Y. K. Kim, S. R. Lee, K. H. Kim, K. H. Cho, H. C. Eun, and J. H. Chung, Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo, J. Invest. Dermatol., 124, 70 (2005).   DOI
13 W. H. Li, Y. M. Lee, J. Y. Kim, S. Kang, S. Kim, K. H. Kim, C. H. Park, and J. H. Chung, Transient receptor potential vanilloid-1 mediates heat-shock-induced matrix metalloproteinase-1 expression in human epidermal keratinocytes, J. Invest. Dermatol., 127, 2328 (2007).   DOI
14 Y. M. Lee, W. H. Li, Y. K. Kim, and J. H. Chung, Heat-induced MMP-1 expression is mediated by TRPV1 through PKC${\alpha}$ signaling in HaCaT cells, Exp. Dermatol., 17(10), 864 (2008).   DOI
15 M. H. Shin, J. E. Seo, Y. K. Kim, K. H. Kim, and J. H. Chung, Chronic heat treatment causes skin wrinkle formation and oxidative damage in hairless mice, Mech. Ageing. Dev., 133(2-3), 92 (2012).   DOI
16 G. Park and M. S. Oh, Acceleration of heat shock-induced collagen breakdown in human dermal fibroblasts with knockdown of NF-E2-related factor 2, BMB. Rep., 48(8), 467 (2015).   DOI
17 T. Taguchi and M. S. Razzaque, The collagen-specific molecular chaperone HSP47 is there a role in fibrosis?, Trends. Mol. Med., 13(2), 45 (2006).
18 Y. Zhang, M. Ghazwani, J. Li, M. Sun, D. B.stolz, F. He, J. Fan, W. Xie, and S. Li, MiR-29b inhibits collagen maturation in hepatic stellate cells through down-regulating the expression of HSP47 and lysyl oxidase, Biochem. Biophys. Res. Commun., 446(4), 940 (2014).   DOI
19 D. Tran, N. Doan, C. Louime, M. Giordano, and S. Portilla, Growth, antioxidant capacity and total carotene of Dunaliella salina DCCBC15 in a low cost enriched natural seawater medium, World. J. Microbiol. Biotechnol., 30(1), 317 (2014).   DOI
20 B. Mogedas, A. Salgueto, C. Casal, and C. Vilachez, Beta-catorotene production enhancement by UV-A radiation in dunaliella bardawil cultivated in laboratory reactors, J. Biosci. Bioeng., 108(1), 47 (2009).   DOI
21 F. F. Madkour and M. M. Abdel-Daim, Hepato-protective and antioxidant activity of Dunaliella salina in paracetamol-induced acute toxicity in rats, Indian. J. Pharm. Sci., 75(6), 642 (2013).