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http://dx.doi.org/10.15230/SCSK.2014.40.4.403

Inhibition of Melanogenesis by Cucurbitacin B from Cucumis sativus L.  

Chang, Yun-Hee (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Choo, Jung-Ha (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Lee, So-Young (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Kim, Tae-Yoon (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Jin, Mu-Hyun (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Chang, Min-Youl (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Lee, Sang-Hwa (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Lee, Cheon-Koo (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Park, Sun-Gyoo (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.4, 2014 , pp. 403-412 More about this Journal
Abstract
To develop an effective skin whitening agent for cosmetics, we isolated cucurbitacin B from Cucumis sativus L. which has been used as traditional skin lighting regimen by the bioactivity-guided fractionation, and investigated the inhibitory effects of cucurbitacin B on melanogenesis. At a non-cytotoxic concentration, cucurbitacin B reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner. Cucurbitacin B did not directly inhibit mushroom tyrosinase activity, but it inhibited intracellular tyrosinase activity in a dose-dependent manner. Its inhibitory mechanism on melanin biosynthesis was further assessed, and we found that cucurbitacin B significantly decreased the protein level of tyrosinase, a major melanogenic enzymes and MITF, a master transcriptional factor of melanogenesis. In addition, cucurbitacin B increased the expression of WW domain-containing oxidoreductase (WWOX) which is known to function as tumor repressor and inhibits $Wnt/{\beta}$-catenin pathway. Collectively, these results suggest that cucuritacin B from C. sativus could be used as an active ingredient for skin whitening.
Keywords
cucurbitacin B; melanogenesis; tyrosinase; mitf; wwox;
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Times Cited By KSCI : 1  (Citation Analysis)
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