Browse > Article
http://dx.doi.org/10.15230/SCSK.2014.40.4.391

Antioxidative Effects and Component Analysis of Extracts of the Rumex acetosa L.  

Jeong, Yoo Min (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Kim, Ho Jae (Hansung Science High School)
Lee, Su Hyun (Hansung Science High School)
Jang, Do Yun (Hansung Science High School)
Choi, Yae Chan (Hansung Science High School)
Min, Na Young (Hansung Science High School)
Gong, Bong Ju (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.4, 2014 , pp. 391-402 More about this Journal
Abstract
In this study, the antioxidative effects and component analysis for the extracts of Rumex acetosa L. were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried R. acetosa L.. Free radical scavenging activities (1,1-diphenyl-2- picrylhydrazyl) size of, in the order of aglycone fraction > ethyl acetate fraction > 50% ethanol extract, aglycone fraction ($45.10{\mu}g/mL$) showed the highest radical scavenging activity. Reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, $OSC_{50}$) on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system was also, in the order of ethyl acetate fraction> aglycone fraction> 50% ethyl acetate fraction, ethyl acetate fraction ($2.68{\mu}g/mL$) was shown a great antioxidant capacity. The total antioxidant capacity of the ethyl acetate fraction was found to be greater than L-ascorbic acid, known as a typical hydrophilic antioxidant ($6.88 {\mu}g/mL$). The cellular protective effects of R. acetosa L. extracts on the $^1O_2$-induced cellular damage of human erythrocytes were exhibited at all concentration-dependent ($1{\sim}25{\mu}g/mL$). Especially, aglycone fraction (${\tau}_{50}$, 104.80 min) in $25{\mu}g/mL$ showed the most protective effect among extracts. Components of the ethyl acetate fraction obtained from R. acetosa L. extracts were analyzed by TLC, HPLC chromatogram, LC/ESI-MS/MS. As a result, the ethyl acetate fraction contained several flavonoids, such as orientin, isoorientin, vitexin, isovitexin. These results indicate that the R. acetosa L. extracts can be used as antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging $^1O_2$ and other ROS. Thus, the extracts of R. acetosa L. could be applicable to new anti-aging cosmeceutical ingredients.
Keywords
R. acetosa L.; antioxidative activity; photoaging; component analysis; cosmeceutical;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 L. Packer, Utraviolet radiation (UVA, UVB) and skin antioxidants, In: Free radical damage and its control, eds. C. A. Rice-Evans and R. H. Burdon, Elsevier Science B.V., 239 (1994).
2 K. Scharffetter-Kochanek, Photoaging of the connective tissue of skin: Its prevention and therapy, In: Antioxidants in disease mechanisms and therapy, eds. H. Sies, Advances Pharmacology, (1997).
3 S. N. Park, Skin aging and antioxidant, J. Soc. Cosmet. Scientists Korea, 23(1), 75 (1997).
4 S. N. Park, Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I), J. Korean Ind. Eng. Chem., 14(5), 657 (2003).   과학기술학회마을
5 S. N. Park, Effect of natural products on skin cells: action and suppression of reactive oxygen species, J. Soc. Cosmet. Scientists Korea, 25(2), 77 (1999).   과학기술학회마을
6 J. R. Kanofsky, H. Hoogland, R. Wever, and S. J. Weiss, Singlet oxygen production by human eosinophils, J. Biol. Chem., 263, 9692 (1988).
7 S. N, Park, Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes, Korean J. Food Sci. Technol., 35(3), 510 (2003).   과학기술학회마을
8 Y. J. Ahn, B. R. Won, M. K Kang, J. H. Kim, and S. N. Park, Antioxidant activity and component analysis of fermented Lavendula angustifolia extracts, J. Soc. Cosmet. Scientists Korea, 35(2), 125 (2009).
9 S. N, Park, Effects of flavonoids and other phenolic compounds on reactive oxygen-mediated biochemical reactions, Ph.D. Thesis, Seoul National University (1989).
10 S. B. Han, S. S. Kwon, B. J. Gong, K. J. Kim, and S. N. Park, Antioxidative Effect and Tyrosinase Inhibitory Activity for the Unripened Fruit by Rubus coreanus Miquel Extracts, J. Soc. Cosmet. Scientists Korea, 39(4), 295 (2013).   DOI
11 R. S. Sohala and W. C. Orrb, The redox stress hypothesis of aging, Free Radic. Biol. Med., 52(3), 539 (2012).   DOI   ScienceOn
12 L. C. Magdalena, and Y. A. Tak, Reactive oxygen species, cellular redox systems, and apoptosis, Free Radic. Biol. Med., 48, 749 (2010).   DOI   ScienceOn
13 V. Afonso, R. Champy, D. Mitrovic, P. l. Collin, and A. Lomri, Reactive oxygen species and superoxide dismutases : Role in joint diseases, Joint Bone Spine, 74, 324 (2007).   DOI   ScienceOn
14 M. J. Davies, Reactive Oxygen Species, Metalloproteinases, and Plaque Stability, Journal of the American Heart Association, 23, 2382 (1998).
15 D. Bagchi, M. Bagchi, E. A. Hassoun, and S. J. Stohs, In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides, Toxicology, 104, 129 (1995).   DOI   ScienceOn
16 S. B. Berman and T. G. Hastings, Inhibition of glutamate transport in synaptosomes by dopamine oxidation and reactive oxygen species, J. Neurochem., 69(3), 1185 (1997).
17 N. J. Lee, J. H. Choi, B. S. Koo, S. Y. Ryu, Y. H. Han, S. I. Lee, and D. U. Lee, Antimutagenicity and cytotoxicity of the constituents from the aerial parts of Rumex acetosa, Biol. Pharm. Bull., 28, 2158 (2005).   DOI
18 J. J. Thiele, C. O. Barland, R. Ghadially, and P. M. Elias, Permeability and antioxidant Barriers in aged epidermis, Skin Aging (B. A. Gilchrest, J. Krutman, eds), Springer-Verlag Berlin Heidelberg, Germany, 65 (2006).
19 J. Y. Bae, Y. S. Rhee, S. Y. Han, E. J. Jeong, M. K. Lee, J. Y. Kong, D. H. Lee, K. J. Cho, H. S. Lee, and M. J. Ahn, A Comparison between Water and Ethanol Extracts of Rumex acetosa for Protective Effects on Gastric Ulcers in Mice, Biomol. Ther., 20(4), 425 (2012).   과학기술학회마을   DOI
20 C. B. Lee, Coloured Flora of Korea I. 246, Hyangmoonsa, Seoul (2003).
21 K. Gescher, A. Hensel, W. Hafezi, A. Derksen, and J. Kuhn, Oligomeric proanthocyanidins from Rumex acetosa L. inhibit the attachment of herpes simplex virus type-1, Antiviral Res., 89, 9 (2011).   DOI
22 M. Wegiera, U. Kosikowska, A. Malm, and H. D. Smolarz, Antimicrobial activity of the extracts from fruits of Rumex species, Cent. Eur. J. Biol., 6, 1036 (2011).   DOI
23 M. Aritomi, I. Kiyota, and T. Mazaki, Flavonoid constituents in leaves of Rumex acetosa, Chem. Pharm. Bull., 13, 1470 (1965).   DOI   ScienceOn
24 T. Kato and Y. Morita, Antraquinone components in Rumex acetosa L., Shoyakugaku Zasshi, 41, 67 (1987).
25 T. Kato, Y. Morita, C-Glycosylflavones with acetyl substitution from Rumex acetosa L., Chem. Pharm. Bull., 38, 2277 (1990).
26 S. N. Park, Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes, Korean J. Food Sci. Technol., 35(3), 510 (2003).   과학기술학회마을
27 J. W. Fairbairn and F. J. El-muhtadi, Chemotaxonomy of anthraquinones in Rumex, Phytochemistry, 11, 263 (1972).   DOI   ScienceOn
28 N. J. Lee, K. H. Lee, D. U. Lee, S. S. Park, Y. H. Han, and S. Y. Ryu, Antimutagenic activity and cytotoxicity of the whole plant of Rumex acetosa, Kor. J. Pharmacogn., 32(4), 338 (2001).   과학기술학회마을