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http://dx.doi.org/10.15230/SCSK.2013.39.1.075

The Effect of Chrysin on the Transcriptional Activity of Vitamin D Receptor in Human Keratinocytes  

Choo, Jung Ha (R&D Center, LG Household & Healthcare, Ltd.)
Lee, Sang Hwa (R&D Center, LG Household & Healthcare, Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.1, 2013 , pp. 75-81 More about this Journal
Abstract
Chrysin (5,7-dihydroxyflavone) is a natural flavonoid found in various plants and foods such as propolis and honey. It has been reported that chrysin has various biological effects including antioxidant, anti-aging, anti-inflammatory and anti-cancer. In this study, we investigated the effect of chrysin on the transcriptional activity of VDR in human epidermal keratinocytes by performing dual-luciferase assay. Chrysin significantly induced the transcriptional activity of VDR in a concentration-dependent manner. The VDR mRNA expression was investigated by quantitative real time PCR and chrysin increased the VDR mRNA expression in normal human epidermal keratinocytes. We also found that chrysin increased the expression of keratinocyte differentiation markers such as keratin 10, involucrin and filaggrin. Therefore, the results suggest that chrysin can stimulate the differentiation of human keratinocytes by increasing transcriptional activity of VDR.
Keywords
chrysin; vitamin D; transcriptional activity; VDR; keratinocyte differentiation;
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