Browse > Article
http://dx.doi.org/10.15230/SCSK.2013.39.1.031

Assessment of Skin Toxicity Using Skin Equivalents Containing Cervi cornus Colla  

Kim, Jandi (Department of Biochemistry, Chung-Ang University College of Medicine)
Li, Hailan (Department of Biochemistry, Chung-Ang University College of Medicine)
Jeong, Hyo-Soon (Department of Biochemistry, Chung-Ang University College of Medicine)
Yun, Hye-Young (Department of Biochemistry, Chung-Ang University College of Medicine)
Baek, Kwang Jin (Department of Biochemistry, Chung-Ang University College of Medicine)
Kwon, Nyoun Soo (Department of Biochemistry, Chung-Ang University College of Medicine)
Choi, Hye-Ryung (Department of Dermatology, Seoul National University College of Medicine)
Park, Kyoung-Chan (Department of Dermatology, Seoul National University College of Medicine)
Kim, Dong-Seok (Department of Biochemistry, Chung-Ang University College of Medicine)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.1, 2013 , pp. 31-38 More about this Journal
Abstract
To substitute animal test, skin equivalents (SEs) have been developed for skin irritation and corrosion test. Recently, we have developed new SEs containing Cervi cornus Colla (CCC). In the present study, we used the SEs for cutaneous cytotoxicity test. Sodium dodecylsulfate (SDS) or sodium carbonate was applied to the SEs-, and the epidermal damage by H&E and immunohistochemical stains was evaluated. Our results showed that SDS or sodium carbonate affected the epidermal part of SEs containing CCC in a dose-dependent manner and decreased the expression of p63. It is concluded that SEs containing CCC could be used for an alternative model of animal test and would be greatly helpful in the development of in vitro irritation and corrosion test.
Keywords
in vitro irritation test; non-animal test; cutaneous cytotoxicity; human skin equivalents; Cervi cornus Colla;
Citations & Related Records
연도 인용수 순위
  • Reference
1 R. H. Rice, and H. Green, Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions, Cell., 18, 681 (1979).   DOI   ScienceOn
2 J. Kim, H. S. Jeong, H. Li, K. J. Baek, N. S. Kwon, H. Y. Yun, H. R. Choi, K. C. Park, and D. S. Kim, Effects of Cervi cornus Colla (deer antler glue) in the reconstruction of a skin equivalent model, Arch. Dermatol. Res., 305, 85 (2013).   DOI   ScienceOn
3 M. A. Perkins, R. Osborne, and G. R. Johnson, Development of an in vitro method for skin corrosion testing, Fundam. Appl. Toxicol., 31, 9 (1996).   DOI   ScienceOn
4 R. Roguet, M. Regnier, C. Cohen, K. G. Dossou, and A. Rougier, The use of in vitro reconstituted human skin in dermotoxicity testing, Toxicol. In Vitro., 8, 635 (1994).   DOI   ScienceOn
5 S. W. Youn, H. J. Cho, I. H. Bae, and K. C. Park, Assessment of skin toxicity using living skin equivalents, Korean. J. Dermatol., 41, 308 (2003).
6 U. F. Schaefer, S. Hansen, M. Schneider, J. L. Contreras, and C.-M. Lehr, Drug Absorption Studies: In Situ, in Vitro, and in Silico Models, eds. C. Ehrhardt and K.-J. Kim, vol. VII, 3, Springer US press, New York (2008).
7 J. H. Draize, E. Alvarez, and M. F. Whitesell, Toxicity and primary irritation of some chemical compounds following oral administration and skin application, Fed. Proc., 5, 174 (1946).
8 C. Augustin, and O. Damour, Pharmacotoxicological applications of an equivalent dermis: three measurements of cytotoxicity, Cell. Biol. Toxicol., 11, 167 (1995).   DOI
9 R. M. Greco, J. A. Iocono, and H. P. Ehrlich, Hyaluronic acid stimulates human fibroblast proliferation within a collagen matrix, J. Cell. Physiol., 177, 465 (1998).   DOI   ScienceOn
10 E. Bell, B. Ivarsson, and C. Merrill, Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro, Proc. Natl. Acad. Sci. U.S.A., 76, 1274 (1979).   DOI   ScienceOn
11 H. J. Cho, I. H. Bae, H. J. Chung, D. S. Kim, S. B. Kwon, Y. J. Cho, S. W. Youn, and K. C. Park, Effects of hair follicle dermal sheath cells in the reconstruction of skin equivalents, J. Dermatol. Scl., 35, 74 (2004).   DOI   ScienceOn
12 C. H. Huh, S. Y. Kim, H. J. Cho, D. S. Kim, W. H. Lee, S. B. Kwon, J. I. Na, and K. C. Park, Effects of mesenchymal stem cells in the reconstruction of skin equivalents, J. Dermatol. Sci., 46, 217 (2007).   DOI   ScienceOn
13 Y. Hijikata, T. Kano, and L. Xi, Treatment for intractable anemia with the traditional Chinese medicines Hominis Placenta and Cervi Cornus Colla (deer antler glue), Int. J. Gen. Med., 2, 83 (2009).
14 Y. E. Kim, S. K. Lee, and U. C. Yoon, Studies on the Components and biological functions of animal hard tissue. Korean. Biochem. J., 6, 13 (1973).
15 C. H. Yao, B. S. Liu, C. G. Liu, and Y. S. Chen, Osteogenic potential using a malleable, biodegradable composite added traditional Chinese medicine: in vitro and in vivo evaluations, Am. J. Chin. Med., 34, 873 (2006).   DOI   ScienceOn
16 G. Pellegrini, E. Dellambra, O. Golisano, E. Martinelli, I. Fantozzi, S. Bondanza, D. Ponzin, F. McKeon, and M. De Luca, p63 identifies keratinocyte stem cells, Proc. Natl. Acad. Sci. U.S.A., 98, 3156 (2001).   DOI   ScienceOn
17 A. B. Truong, M. Kretz, T. W. Ridky, R. Kimmel, and P. A. Khavari, p63 regulates proliferation and differentiation of developmentally mature keratinocytes, Genes. Dev., 20, 3185 (2006).   DOI   ScienceOn