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http://dx.doi.org/10.15230/SCSK.2012.38.4.297

Antioxidative Effect and Tyrosinase Inhibitory Activity of Lindera obtusiloba Blume Extracts  

Won, Doo Hyun (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
Han, Saet Byeol (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
Hwang, Jun Pil (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
Kim, Su Ji (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
Park, Jino (DAEBONG LS)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.38, no.4, 2012 , pp. 297-304 More about this Journal
Abstract
In this study, the antioxidative effects and inhibitory activities on tyrosinase of Lindera obtusiloba Blume (L. obtusiloba Blume) extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of L. obtusiloba Blume were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of ethyl acetate fraction of L. obtusiloba Blume was higher than (+)-${\alpha}$-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities of extract and fractions of L. obtusiloba Blume on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system were similar to L-ascorbic acid, well known as a strong antioxidant. The cellular protective effects of 50 % ethanol extract and ethyl acetate of L. obtusiloba Blume on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner ($1{\sim}25{\mu}g/mL$). Ethyl acetate fraction in $10{\mu}g/mL$ concentration showed the most protective effect among extracts (${\tau}_{50}$ = 361.0 min). The inhibitory effects on tyrosinase of ethyl acetate and aglycone fractions were higher than arbutin, known as a whitening agent. These results indicate that L. obtusiloba Blume extracts can be used as antioxidant, and could be applicable to functional cosmetic ingredient.
Keywords
Lindera obtusiloba Blume; antioxidative activity; tyrosinase; photoaging;
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Times Cited By KSCI : 4  (Citation Analysis)
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