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http://dx.doi.org/10.15230/SCSK.2012.38.1.075

Study on the Relationship between Skin Dryness and Amino Acids in Stratum Corneum  

Joo, Kyung-Mi (R&D Center, Amorepacific Coporation)
Han, Ji-Yeon (R&D Center, Amorepacific Coporation)
Son, Eui-Dong (R&D Center, Amorepacific Coporation)
Nam, Gae-Won (R&D Center, Amorepacific Coporation)
Jeong, Hye-Jin (R&D Center, Amorepacific Coporation)
Lim, Kyung-Min (R&D Center, Amorepacific Coporation)
Cho, Jun-Cheol (R&D Center, Amorepacific Coporation)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.38, no.1, 2012 , pp. 75-82 More about this Journal
Abstract
Natural moisturizing factors (NMFs) are hydrophilic and water-soluble components in stratum corneum of the skin. NMFs absorb water from outer environment and enhance the water-holding capacity of stratum corneum and thereby, prevent the dryness and increase flexibility and plasticity of skin. NMFs are mainly composed of amino acids and their metabolites that are produced from the degradation of filaggrin. Here we established a simultaneous quantification method for 22 amino acids in tape-stripped stratum corneum samples using UPLC-PDA. With this method, we analyzed amino acid contents from tape-stripped stratum corneum samples of forearm and forehead regions from 15 healthy volunteers. Amino acid contents of inner (or upper) region were higher than outer (or lower) region of stratum corneum. Amino acid contents of stratum corneum of forearm were higher by 1.5 fold than forehead region. Of note, total amino acid contents were significantly and inversely correlated with trans-epidermal water loss (TEWL), an index for skin barrier function. Especially, Ser, Glu, Gly, Ala and Thr were determined to positively affect skin mositurizing activities. In conclusion, we could demonstrate that amino acid contents of stratum corneum are closely linked with skin barrier and moisturizing function, providing an important and fundamental methodology for the study of amino acids in skin physiology.
Keywords
amino acids; human stratum corneum; natural moisturizing factor (NMF); UPLC-PDA;
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1 N. Nakagawa, S. Sakai, M. Matsumoto, K. Yamada, M. Nagano, T. Yuki, Y. Sumida, and H. Uchiwa, Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects, J. Invest Dermatol., 122, 755 (2004).   DOI   ScienceOn
2 G. Imokawa, H. Kuno, and M. Kawai, Stratum corneum lipids serve as a bound-water modulator, J. Invest Dermatol., 96, 845 (1991).   DOI   ScienceOn
3 J. P. Sylvestre, C. C. Bouissou, R. H. Guy, and M. B. Delgado-Charro, Extraction and quantification of amino acids in human stratum corneum in vivo, Br. J. Dermatol., 163, 458 (2010).   DOI   ScienceOn
4 I. Horii, Y. Nakayama, M. Obata, and H. Tagami, Stratum corneum hydration and amino acid content in xerotic skin, Br. J. Dermatol., 121, 587 (1989).   DOI   ScienceOn
5 S. Marstein, E. Jellum, and L. Eldjarn, The concentration of pyroglutamic acid (2-pyrrolidone- 5-carboxylic acid) in normal and psoriatic epidermis determined on a microgram scale by gas chromatography, Clin. Chim. Acta., 49, 389 (1973).   DOI   ScienceOn
6 A. V. Rawlings, I. R. Scott, C. R. Harding, et al., Stratum corneum moisturization at the molecular level. J. Invest Dermatol., 103, 731 (1994).   DOI   ScienceOn
7 M. Tanaka, M. Okada, Y. X. Zhen, et al., Decreased hydration state of the stratum corneum and reduced amino acid content of the skin surface in patients with seasonal allergic rhinitis, Br. J. Dermatol., 139, 618 (1998).   DOI   ScienceOn
8 M. Denda, J. Hori, J. Koyama, et al., Stratum corneum sphingolipids and free amino acids in experimentally-induced scaly skin, Arch. Dermatol. Res., 284, 363 (1992).   DOI   ScienceOn
9 S. Kezic, P. Kemperman, E. S. Koster, et al., Lossof- function mutations in the filaggrin gene lead to reduced level of natural moisturizing factor in the stratum corneum, J. Invest Dermatol., 128, 2117 (2008).   DOI   ScienceOn
10 S. Kezic, A. Kammeyer, F. Calkoen, et al., Natural moisturizing factor components in the stratum corneum as biomarkers of filaggrin genotype: evaluation of minimally invasive methods, Br. J. Dermatol., 161, 1098 (2009).   DOI   ScienceOn
11 J. Koyama, I. Horii, K. Kawasaki, et al., Free amino acids of stratum corneum as a biochemical marker to evaluate dry skin, J. Soc. Cosmet. Chem., 35, 183 (1984).
12 M. Watanabe, H. Tagami, I. Horii, et al., Functional analyses of the superficial stratum corneum in atopic xerosis, Arch. Dermatol., 127, 1689 (1991).   DOI   ScienceOn
13 M, Hara, K. Kikuchi, M. Watanabe, et al., Senile xerosis: Functional, morphological, and biochemical studies, J. Geriatr. Dermatol., 1, 111 (1993).
14 C. Katagiri, J. Sato, J. Nomura, et al., Changes in environmental humidity affect the water-holding property of the stratum corneum and its free amino acid content, and the expression of filaggrin in the epidermis of hairless mice, J. Dermatol. Sci., 31, 29 (2003).   DOI   ScienceOn
15 Y. Jokura, S. Ishikawa, H. Tokuda, et al., Molecular analysis of elastic properties of the stratum corneum by solid state 13C-nuclear magnetic resonance spectroscopy, J. Invest Dermatol., 104, 806 (1995).   DOI   ScienceOn
16 P. Caspers, G. Lucassen, E. Carter, et al., In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles, J. Invest Dermatol., 116, 434 (2001).   DOI   ScienceOn
17 G. O'Regan, P. Kemperman, A. Sandilands, et al., Raman profiles of the stratum corneum define 3 filaggrin genotype-determined determined atopic dermatitis endophenotypes, J. Aliergy Clin. Immunol., 126, 574 (2010).   DOI   ScienceOn