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Anti Inflammatory Activity of Viburnum dilatatum Thunb. Extract as Cosmetic Ingredient  

Kwon, Yoo-Bin (R&D Center, COSMAX Inc.)
Yoo, Byoung-Sam (R&D Center, COSMAX Inc.)
Kim, Dae-Shin (Halla Arboretum)
Moon, Seong-Joon (R&D Center, COSMAX Inc.)
Yoon, Moung-Seok (R&D Center, COSMAX Inc.)
Park, Soo-Nam (Research Center for Development of Green Cosmetic, Seoul National University of Science Technology)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.36, no.3, 2010 , pp. 183-191 More about this Journal
Abstract
The crude ethanol extracts and their solvent-partitioned fractions derived from the leaf and twig of Viburnum dilatatum Thunb. were investigated for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging efficacy. The results showed that the butanol-soluble fraction ($SC_{50}\;=\;110.30\;{\mu}g/mL$) exhibited higher anti-oxidant activity than the crude ethanol extract ($SC_{50}\;=\;117.03\;{\mu}g/mL$) in the DPPH assay model. Then, the effects of the same extract samples on the production of nitric oxide were examined in LPS-stimulated RAW264.7 cells. Although the hexane and methylene chloride-soluble fraction showed a weak anti-oxidant activity, they exhibited potent inhibitory activity of NO production above 50 % at a concentration of $10\;{\mu}g/mL$. The hexane-soluble fraction also showed the inhibitory effect on mRNA expression of pro-inflammatory mediators such an TNF-$\alpha$, IL-$1{\beta}$, IL-6, iNOS and COX-2 in LPS-stimulated RAW264.7. These results suggest that the solvent extracts of Viburnum dilatatum Thunb. could be used as an anti-irritation ingredient.
Keywords
Viburnum dilatatum Thunb. extract; SLS; anti-inflammtion; anti-oxidant activity; cytokine;
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