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Analytical Methods on the Determination of Active Ingredients for Hair Nourisher Products  

Kwon, Kyoung-Jin (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Kim, Do-Jung (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Kim, Sang-Seop (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Choi, Yu-Bin (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Kim, Mi-Jeong (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Choi, Myeong-Sin (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Cho, Bo-Kyung (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Kim, Dong-Sup (Cosmetics Research Team, National Institute of Food and Drug Safety Evaluation, KFDA)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.36, no.3, 2010 , pp. 167-174 More about this Journal
Abstract
The hair nourisher products are used for prevention of hair loss and classified as quasi-drug in Korea. As concerns about hair loss has been increased, the demand for hair nourisher products has been growing. It is difficult to analyze their main ingredients because they contain various ingredients including natural plant extracts, vitamins, preservatives and exfoliators. The purpose of this study was to develop and validate simultaneous analytical methods of active ingredients in hair nourisher products such as nicotinamide, tocopherol, salicylic acid, dexpanthenol and benzyl nicotinate by HPLC. The active ingredients were separated on a $C_{18}$ column by using acetonitrile/phosphate buffer as a mobile phase, and detected at UV 220, 270 and 300 nm. The calibration curve showed linearity in the range of $12.5{\sim}800\;{\mu}g/mL$ and the recoveries were 97.3 ~ 103.5 % (RSD 0.9 ~ 2.8 %) in liquid matrix and 101.9 ~ 115.9 % (RSD 0.7 ~ 7.7 %) in shampoo matrix. Validated method was applied to hair nourisher products obtained from distribution market. Fortunately, all samples met their criteria. This study might be expected to provide the method for determining active ingredients in hair nourisher products and lead to promote a rapid market entry.
Keywords
hair nourisher products; quasi-drug; HPLC; simultaneous analytical methods; UV-dioarray;
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1 L. Krcmova, L. Urbanek, D. Solichova, M. Kasparova, H. Vlckova, B. Melichar, L. Sobotka, and P. Solich, HPLC method for simultaneous determination of retinoids and tocopherols in human serum for monitoring of anticancer therapy, J. Sep. Sci., 32(15-16), 2804 (2009).   DOI   ScienceOn
2 N. M. Almeida, J. M. Alves, D. C. Patto, C. R. Lima, J. S. Quenca-Guillen, M. I. Santoro, and E. R. Kedor-Hackmann, Determination of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations by HPLC, Int. J. Cosmet. Sci., 31(6), 445 (2009).
3 A. el-Gindy, F. el-Yazby, A. Mostafa, and M. M. Maher, HPLC and chemometric methods for the simultaneous determination of cyproheptadine hydrochloride, multivitamins, and sorbic acid, J. Pharm. Biomed. Anal., 35(4), 7033 (2004).
4 A. Gentili, F. Caretti, G. D'Ascenzo, S. Marchese, D. Perret, D. Di Corcia, and L. M. Rocca, Simultaneous determination of water-soluble vitamins in selected food matrices by liquid chromatography/ electrospray ionization tandem mass spectrometry, Rapid. Commun. Mass. Spectrom., 22(13), 2029 (2008).   DOI   ScienceOn
5 U. Holler, C. Brodhag, A. Knobel, P. Hofmann, and V. Spitzer, Automated determination of selected water-soluble vitamins in tablets using a bench-top robotic system coupled to reversed-phase (RP-18) HPLC with UV detection, J. Pharm. Biomed. Anal., 31(1), 151 (2003).   DOI   ScienceOn
6 H. J. Jeong, M. H. Lee, K. W. Ro, C. W. Hur, and J. W. Kim, Determination of Panthenol, Cholecalciferol and Tocopherol in Cosmetic Products by Gas Chromatography-Mass Spectrometry in SIM Mode, Int. J. Cosmet. Sci., 21(1), 41 (1999).   DOI   ScienceOn
7 A. U. Kulikov and A. A. Zinchenko, Development and validation of reversed phase high performance liquid chromatography method for determination of dexpanthenol in pharmaceutical formulations, J. Pharm. Biomed. Anal., 19;43(3), 983 (2007).   DOI   ScienceOn
8 D. Siluk, R. V. Oliveira, M. Esther-Rodriguez-Rosas, S. Ling, A. Bos, L. Ferrucci, and I. W. Wainer, A validated liquid chromatography method for the simultaneous determination of vitamins A and E in human plasma, J. Pharm. Biomed. Anal., 44(4), 1001 (2007).   DOI   ScienceOn
9 N. Ramos-Martos, F. Aguirre-Gomez, A. Molina- Diaz, and L. F. Capitan-Vallvey. Application of liquid chromatography to the simultaneous determination of acetylsalicylic acid, caffeine, codeine, paracetamol, pyridoxine, and thiamine in pharmaceutical preparations, J. AOAC Int., 84(3), 676 (2001).
10 B. O. Silva, First derivative spectrophotometric and high performance liquid chromatographic simultaneous determination of benzoic and salicylic acids in pharmaceutical preparations, Nig. Q. J. Hosp. Med., 18(2), 92 (2008).
11 S. Vidovic, B. Stojanovic, J. Veljkovic, L. Prazic Arsic, G. Roglic, and D. Manojlovic, Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method, J. Chromatogr. A., 1202(2), 155 (2008).   DOI   ScienceOn