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Modulatory Effect of Four Azulene Derivatives from the Fruiting Bodies of Lactarius hatsudake on Interferon-$\gamma$ Production  

Xu, Guang Hua (College of Pharmacy, Yanbian University)
Kim, Jae-Wha (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Li, Gao (College of Pharmacy, Yanbian University)
Yoo, Ick-Dong (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.36, no.2, 2010 , pp. 151-156 More about this Journal
Abstract
Investigation of secondary bioactive metabolites from the fruiting bodies of Lactarius hatsudake led to the isolation of four azulene derivatives by means of repeated column chromatography and preparative HPLC, and they were identified as 1-formyl-4-methyl-7-isopropyl azulene (1), lactaroviolin (2), 4-methyl-7-isopropyl-azulene-1-carboxylic acid (3), and 1-formyl-4-methyl-7-(1-hydroxy-1-methylethyl) azulene (4) by their physico-chemical properties and spectroscopic analysis. The isolated compounds were evaluated for the effects on modulation of cytokines in natural killer cell line (NK92 cells). Compounds 1 and 4 strongly inhibited IFN-$\gamma$ production in a dose-dependent manner, corresponding to 101.3 % and 92.7 % inhibition at 400 ${\mu}M$, and 11.9 % and 24.1 % at 100 ${\mu}M$, respectively, whereas compounds 2 and 3 showed weak inhibitory effect on INF-$\gamma$ production, corresponding to 45.9 % and 18.0 % inhibition at 400 ${\mu}M$.
Keywords
Lactarius hatsudake; azulene; interferon-$\gamma$; natural killer cell;
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