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Screening System Establishment for Potential Anti-wrinkle Agents Using Human Fibroblast Elastase  

Oh, Mi-Hee (Department of Biochemistry, College of Medicine, Chung-Ang University)
Lee, Ju-Eun (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, Su-Yeon (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, So-Young (Department of Biochemistry, College of Medicine, Chung-Ang University)
Park, Kyoung-Chan (Department of Dermatology, Seoul National University College of Medicine)
Yun, Hye-Young (Department of Biochemistry, College of Medicine, Chung-Ang University)
Baek, Kwang-Jin (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kwon, Nyoun-Soo (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, Dong-Seok (Department of Biochemistry, College of Medicine, Chung-Ang University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.35, no.1, 2009 , pp. 19-25 More about this Journal
Abstract
It has been reported that not only collagen but also elastin contribute to inhibit skin wrinkle formation. Ultraviolet (UV) radiation induces photo-aging on human skin. Because UV radiation increases elastase activity, it is thought that increased elastase activity could be the major reason for skin elasticity reduction and wrinkle formation by UV. In the present study to standardize elastase activity measuring system, purified elastases from porcine pancrease and human neutrophil, and cell extracts of normal human primary fibroblasts, 3T3 mouse fibroblasts, and CCD-25Sk human fibroblasts were used as various enzyme sources. Furthermore, elastase activities were compared according to concentrations of enzyme and substrate and incubation time. Phosphoramidon was used as a positive control to inhibit elastase activities of normal human primary fibroblasts and CCD-25Sk fibroblasts. However, it had no influence on the activity of porcine pancreatic elastase. Therefore, it is suggested that elastase used for testing anti-wrinkle agents should be selected carefully.
Keywords
human fibroblast; elastase; elastin; aging; wrinkle;
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