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Inhibitory Effects of Tannic acid on Human Skin Fibroblast Elastase Activity  

Lee, Ju-Eun (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, So-Young (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, Su-Yeon (Department of Biochemistry, College of Medicine, Chung-Ang University)
Oh, Mi-Hee (Department of Biochemistry, College of Medicine, Chung-Ang University)
Yun, Hye-Young (Department of Biochemistry, College of Medicine, Chung-Ang University)
Baek, Kwang-Jin (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kwon, Nyoun-Soo (Department of Biochemistry, College of Medicine, Chung-Ang University)
Kim, Dong-Seok (Department of Biochemistry, College of Medicine, Chung-Ang University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.34, no.3, 2008 , pp. 217-223 More about this Journal
Abstract
Elastin is an important component of elastic fibers in the skin. Recently, many studies have reported that elastin is also involved In inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Elastase is a metalloproteinase which acts on degradation of elastin. It is known that elastase activity is increased by ultraviolet (UV) B radiation. Thus, Increased elastase activity could be the major reason for skin elasticity reduction and winkle formation. Tannic acid is a polyphenol found in various fruits and nuts. This molecule has a potent ability to eliminate reactive oxygen species and reactive nitrogen species. In the present study, we investigated whether tannic acid has effects on elastase activity and tropoelastin synthesis. Our results showed that tannic acid reduced elastase activity significantly in a dose-dependent manner. However, the expression of tropoelastin protein and mRNA was not significantly affected by tannic acid. From these results, we suggest that tannic acid may contribute to block tortuosity of elastic fibers by inhibiting elastase. Thus, tannic acid might be developed for a possible agent to Inhibit skin aging.
Keywords
human fibroblast; elastase; elastin; tannic acid; tropoelastin;
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1 I. Ghersetich, P. Teofoli, M. Benci, and T. Lotti, Ultrastructural study of hyaluronic acid before and after the use of a pulsed electromagnetic field, electrorydesis, in the treatment of wrinkles, Int. J. Dermatol., 33, 661 (1994)   DOI   ScienceOn
2 L. Ramanathan and N. P. Das, Inhibitory effects of some natural products on metal-induced lipid oxidation in cooked fish, Biol. Trace Elem. Res., 34, 35 (1992)   DOI
3 E. D. Son, J. Y. Lee, S. Lee, M. S. Kim, B. G. Lee, I. S. Chang, and J. H. Chung, Topical application of 17$\beta$-estradiol increases extracellular matrix protein synthesis by stimulating TGF-$\beta$ signaling in aged human skin in vivo, J. Invest. Dermatol., 124, 1149 (2005)   DOI   ScienceOn
4 E. S. Chan, P. Fernandez, A. A. Merchant, M. C. Montesinos, S. Trzaska, A. Desai, C. F. Tung, D. N. Khoa, M. H. Pillinger, A. B. Reiss, M. Tomic-Canic, J. F. Chen, M. A. Schwarzschild, and B. N. Cronstein, Adenosine A2A receptors in diffuse dermal fibrosis: pathogenic role in human dermal fibroblasts and in a murine model of scleroderma, Arthritis Rheum, 54, 2632 (2006)   DOI   ScienceOn
5 F. Antonicelli, G. Bellon, L. Debelle, and W. Hornebeck, Elastin-elastases and inflamm-aging, Curr. Top. Dev. Biol., 79, 99 (2007)   DOI   ScienceOn
6 N. Fu, Protective effect of tannic acid against TPA- and cigarette smoke condensate-induced DNA strand breaks in human white cells and its antioxidant action, Zhongguo Yi Xue Ke Xue Yuan Xue Bao, 13, 347 (1991)   PUBMED
7 F. Jimenez, T. F. Mitts, K. Liu, Y. Wang, and A. Hinek, Ellagic and tannic acids protect newly synthesized elastic fibers from premature enzymatic degradation in dermal fibroblast cultures, J. Invest. Dermatol., 126, 1272 (2006)   DOI   ScienceOn
8 J. C. Isenburg, D. T. Simionescu, and N. R. Vyavahare, Elastin stabilization in cardiovascular implants: improved resistance to enzymatic degradation by treatment with tannic acid, Biomaterials, 25, 3293 (2004)   DOI   ScienceOn
9 J. Y. Seo, S. H. Lee, C. S. Youn, H. R. Choi, G. E. Rhie, K. H. Cho, K. H. Kim, K. C. Park, H. C. Eun, and J. H. Chung, Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo, J. Invest. Dermatol., 116, 915 (2001)   DOI   ScienceOn
10 G. Godeau and W. Hornebeck, Morphometric analysis of the degradation of human skin elastic fibres by human leukocyte elastase (EC 3-4-21-37) and human skin fibroblast elastase (EC 3-4-24), Pathol. Biol. (Paris), 36, 1133 (1988)   PUBMED
11 R. Homsy, P. Pelletier-Lebon, J. M. Tixier, G. Godeau, L. Robert, and W. Hornebeck, Characterization of human skin fibroblasts elastase activity, J. Invest. Dermatol., 91, 472 (1988)   DOI   ScienceOn
12 K. Tsukahara, Y. Takema, S. Moriwaki, N. Tsuji, Y. Suzuki, T. Fujimura, and G. Imokawa, Selective inhibition of skin fibroblast elastase elicits a concentration- dependent prevention of ultraviolet B-induced wrinkle formation, J. Invest. Dermatol., 117, 671 (2001)   DOI   ScienceOn
13 S. Seite, H. Zucchi, D. Septier, S. Igondjo-Tchen, K. Senni, and G. Godeau, Elastin changes during chronological and photo-ageing: the important role of lysozyme, J. Eur. Acad. Dermatol. Venereol., 20, 980 (2006)   PUBMED
14 N. Tsuji, S. Moriwaki, Y. Suzuki, Y. Takema, and G. Imokawa, The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity, Photochem. Photobiol., 74, 283 (2001)   DOI   ScienceOn
15 M. Hirose, T. Hoshiya, K. Akagi, M. Futakuchi, and N. Ito, Inhibition of mammary gland carcinogenesis by green tea catechins and other naturally occurring antioxidants in female Sprague-Dawley rats pretreated with 7,12-dimethylbenz[$\alpha$] anthracene, Cancer Lett., 83, 149 (1994)   DOI   ScienceOn
16 D. B. Learn and S. J. Moloney, Numbers of murine dermal mast cells remain unchanged during chronic ultraviolet B irradiation, Photodermatol Photoimmunol Photomed., 8, 195 (1991)   PUBMED
17 S. Bunda, N. Kaviani, and A. Hinek, Fluctuations of intracellular iron modulate elastin production, J. Biol. Chem., 280, 2341 (2005)   DOI   ScienceOn
18 Z. Werb, M. J. Randa, J. H. Mckenrow, and R. A. Sandhaus, Elastase and elastin degradation, J. Invest. Dermatol., 79, 154 (1982)   DOI   ScienceOn
19 S. Y. Lee, D. D. Jang, R. M. Ahn, and H. S. Ann, Effects of tannic acid on the skin toxicity and heat shock protein induction by UVB irradiation in hairless mouse, Kor. J. Toxicol., 13, 79 (1997)