Browse > Article

Reliability of In Vitro Assay for Initial Depigmenting Agent Screening  

Nguyen, Dung H. (NRL of Skin Bioactive Material, Department of Biological Engineering, Inha University)
Nguyen, Duc T.M. (NRL of Skin Bioactive Material, Department of Biological Engineering, Inha University)
La, Lyun Hwa (NRL of Skin Bioactive Material, Department of Biological Engineering, Inha University)
Lee, Hyang-Bok (NRL of Skin Bioactive Material, Department of Biological Engineering, Inha University)
Shin, Jung-Hyun (Department of Dermatology, Inha University Hospital)
Kim, Eun-Ki (NRL of Skin Bioactive Material, Department of Biological Engineering, Inha University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.34, no.3, 2008 , pp. 183-188 More about this Journal
Abstract
Initial screening assay for depigmenting agents includes in vitro mushroom tyrosinase assay and antioxidant assay. Based on this screening result, melanin synthesis in melanocyte, in screened samples, is further measured. Measuring cellular melanin needs time, human resource, and skills. Therefore initial screening method should be reliable. We examined, 34 Chinese herbs, correlated the screening assay methods with cellular melanin. No reliable relationship was observed between factors, indicating the limitation in the use of these assays, probably due to the complexicity of melanogenesis.
Keywords
depigmenting agents; screening; mushroom tyrosinase; antioxidant; melanin;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 V. J. Hearing, Biochemical control of melanogenesis and melanosomal organization, J. Investig. Dermatol. Symp. Proc., 4(1), 24 (1999)
2 S. Brigant, E. Camera, and M. Picardo, Chemical and instrumental approaches to treat hyperpigmentation, Pigment. Cell. Res., 16(2), 101 (2003)   DOI   ScienceOn
3 V. P. Zawa and S. T. Mhaskar, Exogenous ochronosis following hydroquinone for melasma, J. Cosmet. Dermato., 3(4), 234 (2004)   DOI   ScienceOn
4 Z. Shaomin, W. Yan, S. M. Ahn, Z. Junyu, W. Ke, Y. Shuxia, J. H. Yeon, and Z. Xuejun, Depigmentation of melanocytes by the treatment of extracts from traditional Chinese herbs: a cell culture assay, Biol. Pharm. Bull., 29(9), 1947 (2006)   DOI   ScienceOn
5 M. S. Blois, Antioxidant determination by the use of a stable free radical, Nature, 181, 1199 (1958)   DOI   ScienceOn
6 N. Agar and A. R. Young, Melanogenesis: a photoprotective response to DNA damage?, Mutat. Res., 571(1), 121 (2004)
7 M. Funayama, H. Arakawa, R. Yamamoto, T. Nishino, T. Shin, and S. Murao, Effects of alphaand beta-arbutin on activity of tyrosinases from mushroom and mouse melanoma, Biosci. Biotechnol. Biochem., 59(1), 143 (1995)   DOI   ScienceOn
8 M. Seiberg, C. Paine, E. Sharlow, P. Andrade- Gordon, M. Costanzo, M. Eisinger, and S. S. Shapiro, Inhibition of melanosome transfer results in skin lightening, J. Invest. Dermatol., 115(2), 162 (2000)   DOI   ScienceOn
9 H. J. Lee and Y. W. Seo, Antioxidant properties of Erigeron annuus extract and its three phenolic constituents, Biotechnol. Bioprocess. Eng., 11(1), 13 (2006)   과학기술학회마을   DOI   ScienceOn
10 D. C. Bennett and M. L. Lamoreux, The color loci of mice - a genetic century, Pigment. Cell. Res., 16(4), 333 (2003)   DOI   ScienceOn
11 J. J. Nordlund, The melanocyte and the epidermal melanin unit: an expanded concept, Dermatol. Clin., 25(7), 271 (2003)
12 M. Tim, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods., 65(1), 55 (1983)   DOI   ScienceOn
13 S. R. Jung, Y. H. Ji, J. H. Kim, and J. K. Hwang, Inhibitory effects of active compounds isolated from Safflower (Carthamus tinctorius L.) seeds for melanogenesis, Biol. Pharm. Bull., 24(12), 1976 (2004)
14 K. Maeda, M. Naganuma, M. Fukuda, J. Matsunaga, and Y. Tomita, Effect of pituitary and ovarian hormones on human melanocytes in vitro, Pigment. Cell. Res., 9(4), 204 (1996)   DOI   ScienceOn
15 R. Kohen and I. Gati, Skin low molecular weight antioxidants and their role in aging and in oxidative stress, Toxicology, 148(2), 149 (2000)   DOI   ScienceOn
16 B. S. Yoo, M. A. Yoo, Y. K. Song, and S. Y. Byun, Regulation of proteins related to melanogenesis by heartwood extract of Morus bombycis using proteome analysis, Biotechnol. Bioprocess. Eng., 12(6), 662 (2007)   과학기술학회마을   DOI   ScienceOn
17 M. Mohorcic, J. Friedrich, I. Renimel, P. Andre, D. Mandin, and J. P. Chaumont, Production of melanin bleaching enzyme of fungal originand its application in cosmetics, Biotechnol. Bioprocess Eng., 12(3), 200 (2007)   과학기술학회마을   DOI   ScienceOn
18 J. V. Schaffer and J. L. Bolognia, The melanocortin- 1 receptor: red hair and beyond, Arch. Dermatol., 137(11), 1477 (2001)   PUBMED
19 T. S. Abudaky, M. T. H. Khanz, M. I. Choudharyx, and S. Oksuz, Potent tyrosinase inhibitors from Trifolium Balansae, Nat. Prod. Res., 20(7), 65 (2006)
20 V. J. Hearing, Biogenesis of pigment granules: a sensitive way to regulate melanocyte function, J. Dermatol. Sci., 37(1), 3 (2005)   DOI   ScienceOn
21 D. S. Kim, S. Y. Kim, S. H. Park, K. Y. Choi, S. B. Kwon, M. K. Kim, J. I. Na, S. W. Young, and K. C. Park, Inhibitory effects of 4-n-butylresorcinol on tyrosinase activity and melanin synthesis, Biol. Pharm. Bull., 28(12), 2216 (2005)   DOI   ScienceOn