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Effects of Esthetic Essential Oils on LPS-Induced Nitric Oxide Generation in Murine Marcrophage RAW 264,7 Cells  

Hong, Jin-Tae (College of Pharmacy, Chungbuk National University)
Lee, Hwa-Jeong (College of Pharmacy, Chungbuk National University)
Lee, Chung-Woo (College of Pharmacy, Chungbuk National University)
Choi, Myoung-Suk (College of Pharmacy, Chungbuk National University)
Son, Dong-Ju (College of Pharmacy, Chungbuk National University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.32, no.2, 2006 , pp. 111-116 More about this Journal
Abstract
Essential oils have been used extensively in pharmacy, medicine, food, beverages, cosmetics, perfumery and aromatherapy. Although anti-bacteria, anti-virus, alleviation of fever operations and an anti-inflammatory properties have been reported, action mechanisms have not been fully discovered. In the present study, anti-inflammatory activities of thirty three essential oils have been evaluated in lipopolysaccharide (LPS)-treated macrophage RAW 264.7 cells by the evaluation of nitric oxide (NO) generation since NO generation is implicated in causal factor of inflammation. Among the tested 33 essential oil, Lemongrass oil showed the most inhibitory effect on LPS-induced NO generation in a dose dependent manner ($IC_{50}$ : $22 {\mu}g/mL$). In further study, it was found that Lemongrass oil inhibited the expression of inducible nitric oxide synthase. These results suggest that Lemongrass oil may be useful for improvements of the inflammatory disease such as pimple acne skin.
Keywords
esesential oil; Lemongrass; nitric oxide; RAW 264.7; iNOS; LPS;
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1 이지은, 이주연, 최점일, 김종관, 김성조, 괴화추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향, 대한치주과학회지, 35 (2005)
2 L. F. Aguirre de Career, Therapeutic use of aromatic substances in al-Andalus, Dynamis, 21, 93 (2001)
3 D. Mlatteliano, Holistic nursing management of pain and suffering.historical view with contemporary application, J. N. Y. State Nurses. Assoc., 34(1), 4 (2003)
4 W. F. Chiou, C. J. Chou, and C. F. Chen, Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages, Life. Sci., 69, 625 (2001)   DOI   ScienceOn
5 F. Tchoumbougnang, P. H Zollo, E. Dagne, and Y. Mekonnen, In viro antimalarial activity of essential oils from Cymbopogon citrates and Ocimum gratissimum on mice infected with Plasmodium berghei, Planta Med., 71(1), 20 (2005)   DOI   ScienceOn
6 J. Cheel, C. Theoduloz, J. Rodriguez, and G. Schrneda-Hirschrnann, Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citrates (DC.) Stapf.), J. Agric. Food. Chern, 53(7) 2511 (2005)   DOI   ScienceOn
7 B. G. Lee, S. H. Kim, O. P. Zee, H. Y. Lee, J. W. Han, and H. W. Lee, Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach, Eur. J. Pharmacol., 406, 301 (2004)
8 변성희, 양재하, 김상찬, 현삼메탄올 추출물이 LPS로 유도된 Raw 264.7 cel1에서의 TNF-$\alpha$, IL-1$\alpha$,IL-6및 nitric oxide 생성에 미치는 영향, 대한본초학회지, 20-7 (2005)
9 S. Cristiane da, S. Silvia, and E. S. Elfrides, LC determination of citral in Cymbopogon citrates volatile oil, J. Piartri Biomed Anal., 37, 597 (2005)   DOI   ScienceOn
10 A. Kumar, Y. Takada, A. M. Boriek, and B. B. Aggarwal, Nuclear factor- KB: its role in health and disease, J. Mol. Med, 82, 434 (2004)
11 J. G. Sea, H. O. Pae, G. S. Oh, K. Y. Chai, Y. G. Yun, T. O. Kwon, and H T. Chung, Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferonand lipopolysaccharide, Gen. Pharmacol., 35, 21 (2000)   DOI   ScienceOn
12 신진영, 박승춘, 김기현, 배춘식, 김성호, 신동호, 김종춘, 랫드에서 레몬그라스 (Cymbopogon citrates) 정유의 급성독성 평가, 한국 실험동물학회지, 21(2), 122 (2005)
13 신경민, 박영미, 김인태, 홍선표, 홍정표, 이경태, Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과, 생약학회지, 34(3), 223 (2003)
14 H. Kawamata, H. Ochiai, N. Mantani, and K. Terasawa, Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line, Am. J. Chin Med, 28, 217 (2000)   DOI   ScienceOn
15 N. Maruyama, Y. Sekimoto, H. Ishibashi, S. Inouye, H. Oshima, H. Yamaguchi, and S. Abe, Supperession of neutrophil accumulation in mice by cutaneous application of geranium essential oil, J. lriflamm (Land)., 2(1), 11 (2005)   DOI   ScienceOn
16 Y. S. Lee, H. S. Kim, S. K. Kim, and S. D. Kim, IL -6 mRNA Exoression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans, J. Microbiol. Biotechnol., 10 (2000)
17 13. W. G. Sea, H O. Pae, G. S. Oh, N. Y. Kim, N. Y. Kim, T. O. Kwon, M. K. Shin, K. Y. Chai, and H. T. Chung, The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW 264.7 macrophages, J. Ethnopharmacol., 76, 119 (2001)   DOI   ScienceOn
18 I. Runnie, M. N. Salleh, S. Mohamed, R. J. Head, and M. Y. Abeywardena, Vasorelaxation induced by common edible tropical plant extracts in isolated rat aorta and mesenteric vascular bed, J. Ethnopharmacol., 92(2-3), 311 (2004)   DOI