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Anti-Oxidative and Inhibitory Effect of Saussurea involucrata on MMP-1 in UVA-irradiated Human Dermal Fibroblast  

Sim, Gwan-Sub (R&D Center, Hanbul Cosmetics Co. Ltd.)
Kim, Jin-Hwa (R&D Center, Hanbul Cosmetics Co. Ltd.)
Na, Young (R&D Center, Hanbul Cosmetics Co. Ltd.)
Lee, Dong-Hwan (R&D Center, Hanbul Cosmetics Co. Ltd.)
Lee, Bum-Chun (R&D Center, Hanbul Cosmetics Co. Ltd.)
Zhang, Yong-He (Department of Pharmacology, School of Basic Medical Science, Peking University)
Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co. Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.31, no.4, 2005 , pp. 329-335 More about this Journal
Abstract
In order to investigate the effects of Saussurea involucrata on (relationship between) aging (and Saussurea involucrata), we examined the activities of antioxidation, in vitro MMP inhibition and UVA-induced MMP-1 expression in human dermal fibroblasts. S. involucrata showed scavenging activities radicals and reactive oxygen species (ROS) with the $IC_{50}$ values of $3.89{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $67.29{\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. At the concentration of $1000{\mu}g/mL$, S. involucrata showed 93.27% inhibition on lipid peroxidation of linoleic acid. S. involucrata inhibited the activities of MMP-1 in a does-dependent manner and the $IC_{50}$ value calculated from semi-log plots was $97.18{\mu}g/mL$. Also, UVA induced MMP expression in human dermal fibroblasts was reduced 42.86% by treatment with S. involucrata, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore S. involucrata was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that S. involucrata might act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.
Keywords
Saussurea involucrata; antioxidation; MMP; human dermal fibroblast; anti-aging;
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