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Studies on the Antioxidative Effect of the Buckwheat (Fagopyrum esculentum Moench) Extract and its Protective Role against Cadmiun-mediated Stress  

Yoon Chang-Soon (Radiant Inc.)
Kim Nam-Hyoung (Radiant Inc.)
Jang Jae-Hyung (Radiant Inc.)
Sang Kyung Jin (Chunchen Bioindustry Foundation)
Ko In Young (Chunchen Bioindustry Foundation)
Choi Shin Wook (Radiant Inc.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.31, no.2, 2005 , pp. 197-206 More about this Journal
Abstract
In the present study, the Chuncheon buckwheat extracts prepared from its seed coats, seeds and stems were used to determine anti-oxidative effects, the content of rutin and phytic acid, and the protective role against cadmium at the cellular level. futhermore, it was evaluated whether the buckwheat, mainly known as a healthy food source, might be applicable to functional cosmetics. Up to $100 {\mu}g/mL$ of the extract was not toxic in HaCaT and B16F10 cell lines using MTT assay. The anti-oxidative capacity of superoxide radicals was shown in seed coats extracts > stem extracts=seed extracts. Although its content of rutin, known as one of effective anti-oxidants, mainly exists in the stem, any extract did not eliminate hydroxyl radicals. Phytic acid, known as a heavy metal-chelate agent, was highly concentrated in the stem. The Chuncheon buckwheat extract had $10\%$ protective effect against the treatment of $50{\mu}M$ cadmium at which $50\%$ of HaCaT cells survived. Confocal laser scanning microscope revealed the intracellular generation of reactive oxygen species (ROS) by cadmium treatment. Finally, we identified that the stem extract had the most protective effect on the elimination of ROS.
Keywords
buckwheat extracts; anti-oxidants; rutin; phytic acid; cadmium;
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