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The Effects of Daecheongryong-tang on Transcription Factors and Adipogenic Genes during 3T3-L1 Differentiation  

Lee, Jun-Moon (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University)
Cho, Sung-Woo (Dept.of Rehabilitation Medicine, College of Oriental Medicine, Dong Eui University)
Kang, Kyung-Hwa (Dept.of Oriental Physiology, College of Oriental Medicine, Dong Eui University)
Lee, Seung-Yeon (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University)
Yu, Sun-Ae (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University)
Publication Information
The Journal of Pediatrics of Korean Medicine / v.24, no.3, 2010 , pp. 92-105 More about this Journal
Abstract
Objectives: The purpose of this study is to investigate the effects of Daecheongryong-tang (DCRT) on the adipogenesis in 3T3-L1 preadipocytes. Methods: 3T3-L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of DCRT ranging 0.25 and 2%. The effect of DCRT on adipogenesis was examined by Oil red O staining, and the protein, RNA, and RT-PCR were measured. Results: Our results showed that DCRT decreased the TG content by ORO staining. To elucidate the mechanism of the effects of DCRT on lowering TG content in 3T3-L1 adipocytes, we examined the DCRT modulate expressions of transcription factors to induce adipogenesis and adipogenic genes which is related to the regulation of accumulation of lipids. As a result, the expression of SREBP1, C/$EBP{\beta}$, C/$EBP{\delta}$, C/$EBP{\alpha}$, and $PPAR{\gamma}$ genes, which induce the adipose differentiation and adipose-specific aP2, adipsin, LPL, CD36, TGF-${\beta}$ and adiponectin genes which regulates fat formations, were decreased. In addition, DCRT reduced the expression of iNOS and IL-6 in 3T3-L1 adipocytes, resulting in inflammation. Conclusions: DCRT could regulate transcript factor related to induction of adipose differentiation, inhibit the accumulation of lipids and expression of the adipogenic genes.
Keywords
Daecheongryong-tang (DCRT); Adipocytes; Differentiation; Transcript factor; Adipogenic genes;
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