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http://dx.doi.org/10.23005/ksmls.2022.7.1.15

Anticancer Effect of Novel Peptide from Abalone (Haliotis discus hannai) based on Next Generation Sequencing Data  

Moon, Hyunhye (3BIGS CO. LTD.)
Hwang-bo, Jeon (3BIGS CO. LTD.)
Veerappan, Karpagam (3BIGS CO. LTD.)
Natarajan, Sathishkumar (3BIGS CO. LTD.)
Chung, Hoyong (3BIGS CO. LTD.)
Park, Junhyung (3BIGS CO. LTD.)
Publication Information
Journal of Marine Life Science / v.7, no.1, 2022 , pp. 15-20 More about this Journal
Abstract
Glioblastoma is one of the highly aggressive central nervous system tumors and it is difficult to treat owing its anatomical location. Peptides are novel class of drugs which has the potential to cross the blood brain barrier and exerts its anti-tumor activity. Here, we discovered a novel peptide from abalone (Haliotis discus hannai) next generation sequencing (NGS) data and tested its anticancer effect on glioblastoma cell line SNU-489. The anticancer activity was measured using a cytotoxicity assay in a time and dose-dependent manner. A concentration and time dependent increase in the cytotoxicity was seen in cells treated with the novel peptide. The highest cytotoxicity rate of about 67% was observed in SNU-489 cells treated with 200 µM peptide for 48 hrs. However, the cytotoxic effect was not or less observed in a normal skin cell line HaCaT at similar concentration, thus, evident of peptide's cell specific anticancer activity. In addition, the gene expression level of necroptosis-related genes was analyzed by qRT-PCR to elucidate the anticancer mechanism of the novel peptide. RIPK3 expression was significantly increased by 9.6-fold in 200 µM of novel peptide treatment group, and MLKL expression level was significantly elevated by 2-fold in 100 µM treated group compared to the control group. Therefore, this study confirmed that the novel abalone-derived peptide has anticancer potency, and it causes cancer cell death through the necroptosis mechanism. Collectively, these results suggest that the novel peptide could be candidate anticancer agent for the treatment of glioblastoma in the future.
Keywords
Haliotis discus hannai; Anticancer; Peptide; Next generation sequencing; Glioblastoma;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Li J, Tong T, Ko D, Chung D, Jeong W, Kim J, Kang S. 2012. Antioxidant and Anti-skin-aging Effects of Abalone Viscera Extracts in Human Dermal Fibroblasts. Korean J Food Preser 19: 463-469.   DOI
2 Oberoi RK, Parrish KE, Sio TT, Mittapalli RK, Elmquist WF, Sarkaria JN. 2016. Strategies to improve delivery of anticancer drugs across the blood-brain barrier to treat glioblastoma. J Neurooncol 18: 27-36.
3 Poon MTC, Bruce M, Simpson JE, Hannan CJ, Brennan PM. 2021. Temozolomide sensitivity of malignant glioma cell lines - a systematic review assessing consistencies between in vitrostudies. BMC Cancer 21: 1240.   DOI
4 Seo J, Go H, Kim C, Nam B, Park N. 2016. Antimicrobial peptide, hdMolluscidin, purified from the gill of the abalone, Haliotis discus. Fish Shellfish Immunol 52: 289-297.   DOI
5 Gong F, Chen M, Zhang Y, Li C, Zhou C, Hong P, Sun S, Qian Z. 2019. A novel peptide from abalone (Haliotis discus hannai) to suppress metastasis and vasculogenic mimicry of tumor cells and enhance anti-tumor effect in vitro. Mar Drugs 17: 244.   DOI
6 Chen J, Liang P, Xiao Z, Chen MF, Gong F, Li C, Zhou C, Hong P, Jung WK, Qian ZJ. 2019. Antiphotoaging effect of boiled abalone residual peptide ATPGDEG on UVB-induced keratinocyte HaCaT cells. Food Nutr Res 63: 3508.
7 Choi S, Seo J, Hwang J, Lee K, Lee I. 2020. Anti-inflammatory Effects of Haliotis discus-derived Antibacterial Peptide (AMP) Ab4-7 as a Functional material for Improving Inflammatory Skin Diseases. J Soc Cosmet Sci Korea 46: 147-157.   DOI
8 De Zoysa M, Nikapitiya C, Whang I, Lee J, Lee J. 2009. Abhisin: a potential antimicrobial peptide derived from histone H2A of disk abalone (Haliotis discus discus). Fish Shellfish Immunol 27: 639-646.   DOI
9 Kim H, Kang S, Kim I, Kim S, Kim D, Ma S, Gao T, Li H, Kim M, Lee T, Ham K. 2006. In vitro Anti-hypertensive, Antioxidant and Anticoagulant Activities of Extracts from Haliotis discus hannai. J Korean Soc Food Sci Nutr 35: 835-840.   DOI
10 Kim C, Go H, Seo J, Park N, Kim G. 2013. Anticancer Mechanism of AP1, Abalone Peptide by Cell Cycle Arrest in Human Gastric Cancer Cells. Cancer Prev Res 18: 33-40.
11 Lee K, Kim J, Veerappan K, Chung H, Natarajan S, Kim K, Park J. 2021. Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against. Insects 12: 466.   DOI
12 Lee SB, Song J, Park A. 2020. A trend of artificial intelligence in the healthcare. The Journal of the Korea Contents Association 20: 448-456.
13 Lim SY. 2014. Cytotoxic and antioxidant activities of abalone (Haliotis discus hannai) extracts. J Life Sci 24: 737-742.   DOI
14 Melo-Lima S, Gajate C, Mollinedo F. 2015. Triggers and signaling cross-talk controlling cell death commitment. Cell Cycle 14: 465-466.   DOI
15 Jung M, Kwon W. 2021. Present Status and Future of AI-based Drug Discovery. J Korea Inst Inf Commun Eng 25: 1797-1808.   DOI
16 Ko S, Lee D, Park W, Yoo J, Yim M, Qian Z, Lee C, Oh J, Jung W, Choi I. 2016. Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells. Int J Mol Med 37: 243-250.   DOI
17 Lee C, Hong K, Chun Y. 2016. The Study on Skin 1st Patch Test of Fermented Hydrolysate from Abalone (Haliotis discus hannaiIno). Journal of the Korea Academia-Industrial Cooperation Society 17: 421-424.
18 Pagano C, Navarra G, Coppola L, Avilia G, Pastorino O, Della Monica R, Buonaiuto M, Torelli G, Caiazzo P, Bifulco M, Laezza C. 2022. N6-isopentenyladenosine induces cell death through necroptosis in human glioblastoma cells. Cell Death Discov 8: 173.   DOI