Browse > Article
http://dx.doi.org/10.23005/ksmls.2021.6.2.66

Antioxidative Activities and Protective Effects on Alcohol-Induced Oxidative Stress in the Human Hepatic HepG2 Cells of Undaria pinnatifida and Costaria costata Extracts  

Kim, Ki An (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation)
Oh, Tae-Hwan (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation)
Chun, Sang-Ho (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation)
Publication Information
Journal of Marine Life Science / v.6, no.2, 2021 , pp. 66-72 More about this Journal
Abstract
We investigated the antioxidant and hepatoprotective effects of extracts from the Undaria pinnatifida and Costaria costata against ethanol-induced oxidative damage. The total polyphenol and flavonoid contents were highest in the 70% ethanol extract from Undaria pinnatifida and Costaria costata. Also, the radical scavenging activity of DPPH (IC50 0.33± 0.21, 0.48±0.47 mg/ml) and ABTS (IC50 0.34±0.30, 0.47±0.17 mg/ml) in the 70% ethanol extract was higher than that of the hot water and 10% ethanol extracts. To determine the hepatoprotective effects of extracts in ethanol-induced oxidative damage, cell viability was measured using an MTT assay. In the pre-treatment of Undaria pinnatifida and Costaria costata hot water extracts, the concentration-dependent increased the cell viability compared with the ethanol treated cells (73.95%) by 89.91~97.63% and 84.99~90.54%, respectively. The data suggests that 70% ethanol extracts have antioxidant activity and hot water extracts exhibit hepatoprotective effects. Therefore, Undaria pinnatifida and Costaria costata may be considered potential agents for control ethanol-induced liver damage.
Keywords
Undaria pinnatifida; Costaria costata; Antioxidative activity; Hepatoprotective effect; Oxidative stress;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Ramadori G, Moriconi F, Malik I, Dudas J. 2008. Physiology and pathophysiology of liver inflammation, damage and repair. J Physiology Pharmacology 59: 107-117.
2 Lee JH. 2005. Antioxidant activity and resistance to reactive oxygen species of Lactobacillus spp. Bifidobacterium spp and Bacillus coagulans. Master's thesis. Chung-Ang University. Seoul, Korea.
3 Choi JS, Bae HJ, Kim YC, Park NH, Kim TB, Choi YJ, et al. 2008. Nutritional composition and biological activities of the methanol extracts of sea mustard (Undaria pinnatifida) in market. J Life Sci 18: 387-394.   DOI
4 Choi JH, Kim IS, Kim JI, Yoon TH. 1992. Studies on anti-aging action of brown algae (Undaria pinnatifida). Bull Korean Fish Soc 25: 181-188.
5 Das D, Pemberton PW, Burrows PC, Gordon C, Smith A, McMahon RFT, Warnes TW. 2000. Antioxidant properties of colchicine in acute carbon tetrachloride induced rat liver injury and its role in the resolution of established cirrhosis. Biochim Biophys Acta 1502: 351-362.   DOI
6 Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. 2004. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52: 794-804.
7 Jung YT. 2012. Antioxidant and liver protection effects of Zizyphus jujuba mill. leaf extracts. Master's thesis. Keimyung University. Daegu, Korea.
8 Kim BM, Jun JY, Park YB, Jeong IH. 2006. Antioxidative activity of methanolic extracts from seaweeds. J Korean Soc Food Sci Nutr 35: 1097-1101.   DOI
9 Kim JH, Kang HM, Lee SH, Lee JY, Park LY. 2015. Antioxidant and a-glucosidase inhibition activity of saeweed extracts. Korean J Food Preserv 22: 290-296.   DOI
10 Kim OK, Lee MH, Kwon HO, Lee DS, Park JJ, Kim EP, You YH, Lim YT, Jun WJ, Lee JM. 2018. Costaria costata extract suppresses development of atopic dermatitis in chloro-2,4-dinitrobenzene-treated NC/Nga mice. Skin Pharmacol Physiol 31: 212-219.   DOI
11 Lee SY, Ahn JW, Hwang HJ, Lee SB. 2011. Seaweed bio-mass resources in Korea. Korean Society for Biotechnology and Bioengineering Journal 26: 267-276.
12 Ohshima H, Tazawa H, Sylla BS, Sawa T. 2005. Prevention of human cancer by modulation of chronic inflammatory processes. Mutat Res 591: 110-122.   DOI
13 Kim TD, Song HI, Hong JP, Jeon CY, Kim SK, Han HK, Kim DS, Bang JD. 2006. Growth and maturation of the brown seaweed Costaria costata transplanted for the wildstock enhancement. Journal of Life Science 16: 1044-1051.   DOI
14 Kamel KM, El-Raouf OMA, Metwally SA, El-Latif HAA, ElSayed ME. 2014. Hesperidin and rutin, antioxidant citrus flavonoid, attenuate cisplatin-induced nephrotoxicity in rats. J Biochem Mol Toxicol 28: 312-319.   DOI
15 Kim KH, Kim CS. 1982. Studies on the manufacture of Undaria pinnatifida, laver and it's physiocochemical properties. Korean J Food Sci Tech 14: 336-341.
16 Kim S, Woo S, Yun H, Yum S, Choi E, Do J-R, Jo J-H, Kim D, Lee S, Lee TK. 2005. Total phenolic contents and biological activities of Korean seaweed extracts. Food Sci Biotechnol 14: 798-802.
17 Lim CW, Park HY, Shim KB, Yoon NY, Kim YK. 2012. Anti-angiogenesis activity and characterization of extract of Ark Shell Scapharca subcrenata. Kor J Fish and Acua Sci 45: 303-306.
18 Middleton E, Kandaswami C. 1994. Potential healthpromoting properties of citrus flavonoids. Food Technol 48: 115-119.
19 Papa S, Bubici C, Zazzeroni F, Franzoso G. 2009. Mechanisms of liver disease, cross-talk between the NF-kappaB and JNK pathways. Biol Chem 390: 965-976.   DOI
20 Na YJ, Jeon DV, Han SJ, Maranguy A, An DS, Cha HK, Lee JB, Yang JH, Lee HW, Choi HG. 2016. Crossed effects of light and temperature on the growth and maturation of gametophytes in Costaria costata and Undaria pinnatifida. Korean J Fish Aquat Sci 49: 190-197.   DOI
21 Park JL, Chae KY, Hong JS. 2007. A comparison of antioxidant activities in black sesame seeds according to preparation and cooking conditions. J East Asian Soc Dietary Life 17: 520-531.
22 Ramchandani VA, Bosron WF, Li TK. 2001. Research advances in ethanol metabolism. Pathologie Biologie 49: 676-682.   DOI
23 Vijaya K, Ananthan S, Nalini R. 1995. Antibacterial effect of theaflavin, polyphenon 60 (Camellia sinensis) and Euphorbia hirta on Shigella spp. a cell culture study. J Ethnopharmacol 49: 115-118.   DOI
24 Shim JM, Ahn BG, Kan CW. 2003. The neutral characteristic and effect of brown algae as functional substances in poultry. Korean Soc Poultry Sci 16: 38-52.
25 Park KE, Jang M-S, Lim CW, Kim Y-K, Seo Y, Park H-Y. 2005. Antioxidant activity on ethanol extract from boiled-water of Hizikia fusiformis. J Korean Soc Appl Biol 48: 435-439.
26 Park JH, Kweon GR. 2013. Clinical applications of antioxidants. Han-yang Med Rev 33: 130-136.   DOI
27 Rouach H, Clement M, Orfanelli MT, Janvier B, Nordmann J, Nordmann R. 2005. Hepatic lipid peroxidation and mitochondrial susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochimica et Biophysica Acta 753: 439-444.   DOI
28 Ferreres F, Lopes G, Gil-Izquierdo A, Andrade PB, Sousa C, Mouga T, Valentao P. 2012. Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MSN: Approaches to hyaluronidase inhibitory capacity and antioxidant properties. Mar Drugs 10: 2766-2781.   DOI