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http://dx.doi.org/10.14406/acu.2021.027

Combined Effects of Different Wavelength Laser Stimulations and Electroacupuncture on Neuropathic Pain  

Cho, Myoung-rae (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Dong-shin University)
Kim, Myeong-hun (Department of Meridian & Acupoint, College of Korean Medicine, Dong-shin University)
Moon, Youngmin (Smart Solution Research Group, Research Institute of Industrial Science and Technology)
Jeong, Sungho (Department of Mechatronics, Gwangju Institute of Science Technology)
Na, Chang-su (Department of Meridian & Acupoint, College of Korean Medicine, Dong-shin University)
Publication Information
Korean Journal of Acupuncture / v.38, no.3, 2021 , pp. 151-161 More about this Journal
Abstract
Objectives : The effects of a combined stimulation of 658 nm, 830 nm, 904 nm, and 1064 nm laser acupuncture treatment (LAT) and electroacupuncture treatment (EAT) on GB39 and GB34 on neuropathic pain in rats induced by tibial and sural nerve transection were studied in this paper. Methods : To express a neuropathic pain model, surgery was performed to transection rats' tibial and sural nerves. The rats were divided into normal group, control group, and experimental groups. In addition, the experimental groups were divided into 658 nm laser and electroacupuncture (LAT658+EAT), 830 nm laser and electroacupuncture (LAT830+EAT), 904 nm laser and electroacupuncture (LAT904+EAT), and 1064 nm laser and electroacupuncture (LAT830+EAT). For the treatment of the experimental groups, electroacupuncture and different laser wavelengths were alternately applied to GB34 and GB39 twice a week for 3 weeks for 1 minute 30 seconds. The withdrawal response of neuropathic rats' legs by acetone stimulation was observed, as well as the c-Fos in the central gray region in the midbrain of neuropathic rats together with Bax, Bcl2, and mGluR5 expressions associated with apoptosis. Results : Compared with the control group, a significant decrease in the frequency of paw withdrawal in response to acetone allodynia was observed in LAT658+EAT and LAT830+EAT groups in 6th times, LAT904+EAT group in 2nd, 3rd, and 6th times, and LAT1064+EAT group in 2nd and 6th times, respectively. For c-fos positive cells in the central gray region, a significant decrease was observed in LAT830+EAT, LAT904+EAT, and LAT1064+EAT groups in comparison with the control group. In Bax expression, LAT1064+EAT group showed a significant decrease compared to the control group. In Bcl-2 expression, the LAT658+EAT,the LAT904+EAT, and the LAT1064+EAT groups increased significantly compared to the control group. LAT830+EAT, LAT904+EAT, and LAT1064+EAT groups showed significantly increased mGlu5 expression compared to the control group. Conclusions : The combination of laser for each wavelength and electroacupuncture alternately performed in this study is thought to be effective in improving neuropathic pain and apoptosis.
Keywords
neuropathic pain; 658 nm.830 nm.904 nm.1064 nm laser; electroacupuncture; acetone allodynia; c-Fos; apoptosis;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Hayashida K, Obata H, Nakajima K, Eisenach JC. Gabapentin acts within the locus coeruleus to alleviate neuropathic pain. Anesthesiology. 2008 ; 109(6) : 1077-84. https://doi.org/10.1097/ALN.0b013e31818dac9c   DOI
2 Bullitt E. Expression of c-fos like protein as a marker for neuronal activity following noxious stimulation in the rat. J Comp Neurol. 1990 ; 296(4) : 517-30. https://doi.org/10.1002/cne.902960402   DOI
3 Nanjing University of Chinese Medicine. The an-notation of The Huang Di Nei Jing-So Moon. Shanghai : Shanghai Scientific and Technical Publishers. 1997 : 278, 361, 305-12, 652.
4 Textbook Compilation Committee of the Korean Society of Acupuncture and Medicine. Acupuncture Medicine. Seoul. Hanmi Publishing. 2020 : 142-50, 199-202.
5 Min JH, Park CM, Moon DE, Kim SN, Chung CW, Kim KH. Fos expression in the brain of neuropathic pain rats. Korean J Anesthesiol. 2001 ; 41(2) : 229-38. http://doi.org/10.4097/kjae.2001.41.2.229   DOI
6 Gibson ME, Han BH, Choi J, Knudson CM, Korsmeyer SJ, Parsadanian M, Holtzman DM. BAX contributes to apoptoticlike death following neonatal hypoxia-ischemia: evidence for distinct apoptosis pathways. Mol Med. 2001 ; 7 : 644-55.   DOI
7 Kim HK, Kim PJ, Baik SW, Kim IS, Chung KS. Molecular Biologic Study on the Changes of Glutamate Receptor (mGluR5) in Rat Hippocampus after Brain Ischemia. Korean J Crit Care Med. 2000 ; 15(2) : 75-81.
8 Gross A, McDonnell JM, Korsmeyer SJ. Bcl-2 family members and the mitochondria in apoptosis. Genes Develop. 1999 ; 13 : 1899-911.   DOI
9 Zhao L, Jiao Q, Yang P, Chen X, Zhang J, Zhao B, et al. Metabotropic glutamate receptor 5 promotes proliferation of human neural stem/progenitor cells with activation of mitogen-activated protein kinases signaling pathway in vitro. Neuroscience. 2011 ; 192 : 185-94. https://doi.org/10.1016/j.neuroscience.2011.06.044   DOI
10 Zhang JY. Lei Jing (shang). Seoul : Daesung Publisher. 1982 : 372-5.
11 Bang SH, You DH, Cho MR, Yim HJ, Na CS. Effects of Invasive Low Level Laser Acupuncture Therapy (LLLAT) depending on capacity at LU7+LR5 on the Hyperlipemia Rats Induced by High Fat Diet. Korean J Acupunct. 2006 ; 23(1) : 1-14.
12 Jan T, Lars H (Jang IS, Shin GB). Laser Therapy. Seoul : Jeong-Dam Publishing. 2006 ; 107.
13 Wei TS, Youn DH, Youn YC, Na CS. Comparative study of acupuncture and invasive laser acupuncture therapy at SI3.BL40 on the tibial, sural nerve injury and L5 spinal nerve ligation model in rats. Korean J Acupunct. 2005 ; 22(2) : 9-24.
14 Ihm SJ, Youn DH, Na CS. Effects of Invasive Low Level Laser Acupunc- ture Therapy (LLLAT) at LU7, LR5, LU7+LR5 on the Hyperlipemia Rats Induced by High Fat Diet. Korean J Acupunct. 2005 ; 22(3) : 1-16.
15 Johnson Jr EM, Greenlund LJ, Akins PT, Hsu CY. Neuronal apoptosis: current understanding of molecular mechanisms and potential role in ischemic brain injury. J Neurotrauma. 1995 ; 12 : 843-52. https://doi.org/10.1089/neu.1995.12.843   DOI
16 Na CS, Youn DH, Choi CH, Lee SH, Oh KH, Jeong SH. The effects of traditional acupuncture techniques and green laser acupuncture on the blood pressure in hypertensive rat induced by two kidney one clip. Korean J Acupunct. 2008 ; 25(2) : 199-210.
17 Kim YS , Lee JH, Lee SH, Na CS, Cho MR. Effects of Electro and Laser Acupuncture Treatment with GB39 and GB34 on Neuropathic Pain in Rats Induced by Tibial and Sural Nerve Ligation. J Acupunct Res. 2013 ; 30(3) : 125-34. https://doi.org/10.13045/acupunct.2013012   DOI
18 Youn D, Youn P, Kim W, Kim M, Choi D, Na C. Effects of Laser Acupuncture Therapy According to the Wavelength at GB34.GB39 on Neuropathic Pain Rat Induced by Tibial and Sural Nerve Transection. Korean J Acupunct. 2014 ; 31(4) : 195-207. https://doi.org/10.14406/acu.2014.038   DOI
19 Tuner J, Hode L. Low Level Laser Therapy: Clinical Practice and Scientific Background. Grangesberg Sweden : Prima Books. 1999 : 1-9.
20 Lee BH, Sohn JH, Park YG. Effectiveness of Opioids in the Management of Neuropathic Pain. The Journal of Korean Brain Society. 2001 ; 1(1) : 53-64.
21 The Meridian & Acupuncture Points Textbook Compilation Committee. Acupuncture Points. Seoul : Jeongdam Publishing. 2020 : 346-50, 364-8.
22 Okazaki R, Namba H, Yoshida H, Okai H, Miura T, Kawamura M. The antiallodynic effect of Neurotropin is mediated via activation of descending pain inhibitory systems in rats with spinal nerve ligation. Anesth Analg. 2008 ; 107(3) : 1064-9. https://doi.org/10.1213/ane.0b013e31817e7a59   DOI
23 Heavner JE, Calvillo O, Racz GB. Thermal grill illusion and complex regional pain syndrome type I (reflex sympathetic dystrophy). Reg Anesth. 1997 ; 22(3) : 257-9. https://doi.org/10.1016/S1098-7339(06)80011-8   DOI
24 Dubner R, Ruda MA. Activity-dependent neuronal plasticity following tissue injury and inflammation. Trends Neurosci. 1992 ; 15(3) : 96-103. https://doi.org/10.1016/0166-2236(92)90019-5   DOI
25 Crowe MJ, Bresnahan JC, Shuman SL, Masters JN, Beattie MS. Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med. 1997 ; 3 : 73-6.   DOI
26 Seltzer Z, Shir Y. Sympathetically-maintained causalgiform disorders in a model for neuropathic pain: a review. J Basic Clin Physiol Pharmacol. 1991 ; 2(1-2) : 17-61. https://doi.org/10.1515/jbcpp.1991.2.1-2.17   DOI
27 Song IS, Yang JY, Lee JK, Joo YB, Cha SM. Variations of Neurotrophic Factors and It's Importances in Spinal Cord Injured Rats and Beagle Dogs. J Korean Soc Spine Surg. 2011 ; 18(1) : 1-12. https://doi.org/10.4184/jkss.2011.18.1.1   DOI
28 McMahon SB. Mechanism of sympathetic pain. Br Med Bull. 1991 ; 47(3) : 584-600. https://doi.org/10.1093/oxfordjournals.bmb.a072494   DOI
29 Sato J, Perl ER. Adrenergic excitation of cutaneous pain receptors induced by peripheral nerve injury. Science. 1991 ; 251(5001) : 1608-10. https://doi.org/10.1126/science.2011742   DOI
30 Jang Y, Moon DE, Yoo JH, Lee HJ, Lee JY, Kwon OK. Effects of Oxcarbazepine on Mechanical and Cold Allodynia in a Neuropathic Rat Model. Korean J Anesthesiol. 2003 ; 45(3) : 385-92. http://doi.org/10.4097/kjae.2003.45.3.385   DOI