Browse > Article
http://dx.doi.org/10.14406/acu.2020.007

Inhibitory Effect of Polyporus umbellatus Extract on Melanogenesis  

Kang, Lea Minju (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Park, Seol-a (Department of Beauty Design, Graduate School, Wonkwang University)
Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Research Center of Environmental Science, Wonkwang University)
Publication Information
Korean Journal of Acupuncture / v.37, no.1, 2020 , pp. 24-30 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate melanogenesis inhibition of ethanol extract of Polyporus (EP) by using B16F10 melanoma cells. Methods : We measured antioxidant effect of EP by using 1,1-Diphenyl-1-picrylhydrazyl (DPPH) assay and we confirmed melanin contents and tyrosinase activity of EP in cells. Additionally, the expression of tyrosinase-related protein-1 (TRP-1) and TRP-2 was observed by Western blot. Results : EP showed significantly high radical scavenging activity and inhibition of melanogenesis in dose-dependent manner by decreasing cellular tyrosinase activity and melanin content with or without α-melanin stimulating hormone. TRP-1 and TRP-2 expressions were also suppressed by EP in B16F10 cells. Conclusions : These results suggest that EP inhibits the melanogenesis and it could be a new organic ingredient for hyper-pigmentation.
Keywords
Polyporus; tyrosinase activity; melanin; tyrosinase-related protein-1; tyrosinase-related protein-2; ${\alpha}$-melanin stimulating hormone;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Jang TW, Park JH. Evaluation of whitening activities of flower and fruit of Sophora japonica L. Korean J Plant Res. 2017 ; 30(1) : 22-8. https://doi.org/10.7732/kjpr.2016.30.1.022   DOI
2 Yu JS, Kim AK. Effect of combination of taurine and azelaic acid on antimelanogenesis in murine melanoma cells. J Biomed Sci. 2010 ; 17 Suppl 1: S45. https://doi.org/10.1186/1423-0127-17-s1-s45   DOI
3 Yamaguchi Y, Brenner M, Hearing VJ. The regulation of skin pigmentation. J Biol Chem. 2007 ; 282(38) : 27557-61. https://doi.org/10.1074/jbc.R700026200   DOI
4 Hwang BH. Medicinal Mushrooms mushrooms used in Traditional Oriental Medicine. Forest Bioenergy. 2008 ; 27(1): 36-46.
5 Jin JP, Jang JS. Edible medicinal herbal dictionary. 1st ed. Seoul : Bubinprint. 2013 : 880.
6 Kaminage T, Yasukawa K, Kanno H, Tai T, Nunoura Y, Takido M. Inhibitory effects of lanostane-type triterpene acids, the components of Poria cocos, on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin. Oncology. 1996 ; 53(5) : 382-5. https://doi.org/10.1159/000227592   DOI
7 Lee WY, Park Y, Park Y, Ahn JK, Park SY, Lee HJ. Cytotoxic activity of ergosta-4,6,8(14),22-tetraen-3-one from the sclerotia of Polyporus umbellatus. Bull Korean Chem Soc. 2005 ; 26(9) : 1464-6.   DOI
8 Lu W, Adachi I, Kano K, Yasuta A, Toriizuka K, Ueno M, et al. Platelet aggregation potentiators from cho-rei. Chem Pharm Bull (Tokyo). 1985 ; 33(11): 5083-7. https://doi.org/10.1248/cpb.33.5083   DOI
9 Ishida H, Inaoka Y, Shibatani J, Fukushima M, Tsuji K. Studies of the active substances in herbs used for hair treatment. II. Isolation of hair regrowth substances, acetosyringone and polyporusterone A and B, from Polyporus umbellatus fries. Bio Pharm Bull. 1999 ; 22(11) : 1189-92. https://doi.org/10.1248/bpb.22.1189   DOI
10 Ohsawa T, Yukawa M, Takao C, Murayama M, Bando H. Studies on constituents of fruit body of Polyporus umbellatus and their cytotoxic activity. Chem Pharm Bull (Tokyo). 1992 ; 40(1) : 143-7. https://doi.org/10.1248/cpb.40.143   DOI
11 Park JC, Yoo BG. The effects of inhibited on superoxide radical to Grifola umbellata extract on mice. Journal of radiological science and technology. 2003 ; 26(4) : 47-52.
12 Nguyen TPT, Hung TM, Cuong TD, Huh JI, Kwack SJ, Kim JM, et al. Analysis and stability test of the extract from Ephedrae herba, Phytolaccae radix and Polyporus for toxicity study. Korean J Pharmacogn. 2010 ; 41(2) : 141-6.
13 Lee HS, Hwang IH, Kim JA, Choi JY, Jang TS, Osada H, et al. Isolation of protein tyrosine phosphatase 1B inhibitory constituents from the sclerotia of Polyporus umbellatus fries. Bull Korean Chem Soc. 2011 ; 32(2) : 697-700.   DOI
14 Lee JW, Shin YJ, Cho DJ, Lim HJ, Choi WE, Lee YK. Antitumor and antimutagenic effect of the proteinpolysaccharides from Polyporus umbellatus. J Korean Soc Food Sci Nutr. 2004 ; 33(3) : 475-9. https://doi.org/10.3746/jkfn.2004.33.3.475   DOI
15 Ha YD. Antitumoral, Antioxidant and antimicrobial activities of solvent franctions from Grifola umbellatus. Korean J Postharvest Sci Technol. 2001 ; 8(4) : 481-7.
16 Park JC. The radioprotective effects of Grifola umbellata hot water extract on mice. Journal of radiological science and technology. 2005 ; 28(4) : 333-40.
17 Hong YJ, Jang AR, Yang KS. Inhibition of melanin production and tyrosinase expression of ergosterol derivatives from Phellinus pini. Nat Prod Sci. 2013 ; 19(3) : 258-62.
18 Cho YK, Shin DS, Paik DH, Yoon DY, Shin D. Tyrosinase inhibitory effect of 3,4-dihydroxybenz aldehyde Isolated from Pinellia ternate. Kor J Herbology. 2006 ; 21(2) : 1-7.
19 Blois M. Antioxidant determinations by the use of a stable free radical. Nature. 1958 ; 181: 1199-200. https://doi.org/10.1038/1811199a0   DOI
20 Martinez-Esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC. Mechanism of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse Mmlanoma cells. Eur J Biochem. 1998 ; 255 : 139-46.   DOI
21 Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by $1{\alpha}$, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 1985 ; 45 : 1474-8.
22 Videira, IFS. Moura DFL, Magina S. Mechanisms regulating melanogenesis. An Bras Dermatol. 2013 ; 88(1) : 76-83. https://doi.org/10.1590/s0365-05962013000100009   DOI
23 Matta MK, Zusterzeel R, Pilli NR, Patel V, Volpe DA, Florian J, et al. Effect of sunscreen application under maximal use conditions on plasma concentration of sunscreen active ingredients: a randomized clinical trial. JAMA. 2019 ; 321(21) : 2082-91. https://doi.org/10.1001/jama.2019.5586   DOI
24 Tan XL, Lei G, Wang GH. Polyporus umbellatus inhibited tumor cell proliferation and promoted tumor cell apoptosis by downregulating AKT in breast cancer. Biomed Pharmacother. 2016 ; 83 : 526-35. https://doi.org/10.1016/j.biopha.2016.06.049   DOI