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http://dx.doi.org/10.14406/acu.2019.002

Toxicity Evaluation of TA, a Pharmacopuncture Medicine, in an in Vivo Micronucleus Test  

Hwang, Ji Hye (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University)
Jung, Hyo Won (Department of Herbology, College of Korean Medicine, Dongguk University)
Jung, Chul (Namsangcheon Korean Medicine Clinic)
Publication Information
Korean Journal of Acupuncture / v.36, no.1, 2019 , pp. 74-80 More about this Journal
Abstract
Objectives : TA, a polyherbal extract, typically is used for pharmacopuncture therapy on patients with traffic accident-related injuries and musculoskeletal diseases. This study was performed to evaluate the safety of the TA extract, using a micronucleus test. Methods : The dose range and sampling time were first established. An in vivo micronucleus test was then performed to determine the induction of micronuclei in mouse bone marrow cells after a single intramuscular administration of TA to 7-week-old ICR mice (0.2 ml/animal, at 24 hours post-dosing). Results : The incidence of micro-nucleated polychromatic erythrocytes (PCEs) in PCEs in the TA group was similar to that in the negative-control group, while that in the positive-control group was significantly greater. The positive- and negative-control groups did not differ in the ratio of PCEs to total erythrocytes. Conclusions : Our toxicity study indicates that the TA extract does not induce micronucleus formation in mouse bone marrow cells.
Keywords
micronucleus; pharmacopuncture; safety; TA extract; toxicity test;
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