Browse > Article
http://dx.doi.org/10.14406/acu.2017.008

The Effect of Lactobacillus Mixture Culture Fluid Extracts on Atopic Dermatitis Chemokine Expression of in HaCaT Cells  

Hong, Soo-Jeong (Department of Health Care Management Administration, Graduate School of Business Administration, Gachon University)
Lee, Won-Jae (Department of Health Care Management Administration, Graduate School of Business Administration, Gachon University)
Jo, Eul-Hwa (Department of Meridians and Acupoints, College of Korean Medicine, Wonkwang University)
Ahn, Seong-Hun (Department of Meridians and Acupoints, College of Korean Medicine, Wonkwang University)
Publication Information
Korean Journal of Acupuncture / v.34, no.2, 2017 , pp. 82-87 More about this Journal
Abstract
Objectives : Recently the case of lactobacillus mixture culture fluid appliment was reported. In this study, anti-inflamation effects and anti-allergy effects were studied by stimulus of lactobacillus mixture culture fluid extracts in HaCaT cells. Methods : The atopic dermatitis were induced by TNF-${\alpha}$ and interferon-${\gamma}$ in HaCaT cells. TARC/CCL17, MDC/CCL22, RANTES/CCL5 and ROS production were investigated to explain anti-inflamation and allergy effects of lactobacillus mixture culture fluid with cell-enzyme-linked Immunosorbent assay in 450 nm, 485 nm, 535 nm with spectro-fluorometer. Results : The extracts of lactobacillus mixture culture fluid were decreased TARC/CCL17, MDC/CCL22, RANTES/CCL5 expressions and ROS production with a concentration dependent manner. Conclusions : The effects mechanism of Lactobacillus mixed culture fluid for atopic dermatitis symptoms were considered to be explain anti-inflamation and allergy effects via control of cytokine, chemokine and ROS production, and the fluid could be applied in skin cells directly. But classified AD symptom degrees reported in clinical case before as Reaction Period, Reduction Period, Effect Period, Reproduction Period and Rebound Period could not be explained. Further study will be expected.
Keywords
atopic dermatitis; lactobacillus mixted culture fluid; HaCaT cell; TARC/CCL17; MDC/CCL22; RANTES/CCL5;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Jo EH, Kim TK, Hong SJ, Jung DY, Hwang SY, Ahn SH. The Case Study of Lactobacillus mixture culture fluid on Atopic dermatitis. J Koean Med. 2015 ; 36(3) : 135-43.   DOI
2 Campbell JJ, Haraldsen G, Pan J, Rottman J, Qin S, Ponath P, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 1999 ; 400 : 776-80.   DOI
3 Song TW, Kwon BC, Choi SY, Shin YH, Lee KE, Yang HS et al. Increased Serum Thymus and Activation-regulated Chimokine (TARC) Levels in Children with Atopic Dermatitis. Allergy Asthma & Respiratory Disease. 2005 ; 15(3) : 250-6.
4 Andrew D Luster. The role of chemokines in linking innate and adaptive immunity. Curr Opin Immunol. 2002 ; 14(1) : 129-35.   DOI
5 Howlett S. Emotional dysfunction, child-family relationships and childhood atopic dermatitis. Br J Dermatol 1999 ; 140 : 381-4.   DOI
6 Foolad N, Brezinski EA, Chase EP. Armstrong AW(2012) Effect of nutrient supplementation on atopic dermatitis in children. Arch Dermatol. 2012 ; 17 : 1-6.
7 Hwang EK. The Clinical Significance of Fungal Allergy in Patients with Atopic Dermatitis. Ajou:Department of Medical Sciences, The Graduate School, Ajou University. Paper of master degree. 2012 : 1-34.
8 David TJ, Wells FE, Sharpe TC, Gibbs AC. Low serum zinc in children with atopic eczema. Br J Dermatol 1984 ; 111 : 597-601.   DOI
9 Searing DA, Leung DY. Vitamin D in atopic dermatitis, asthma and allergic diseases. Immunol Allergy Clin North Am. 2010 ; 30 : 397-409.   DOI
10 Leung DY, Bieber T. Atopic dermatitis. Lancet. 2003 ; 361 : 151-60.   DOI
11 Schults-Larsen F, Hanifin JM. Epidermiology of atopic dermatitis. Immunol Allergy Clin. North Am. 2002 ; 22 : 1-24.
12 Soma Y, Kashima M, Imaizumi A, Takahama H, Kawakami T, Mizoguchi M. Moisturizing effects of topical nicotinamide on atopic dry skin. Int J Dermatol. 2005 ; 44 : 197-202.   DOI
13 Park TM. Effect of The Herbal Medicine Treatment for Gastrointestinal Dysfuction in Children with Atopic Dermatitis. Pocheon: Major in Comlementary Alternative Medicine Graduate School of Complementary Alternative Medicine. paper of master degree. 2008 : 1-43.
14 Pessi T, Sutas Y, Hurme M, Iolauri E. Interleukin-10 generation in atopic children following oral Lactobacillus rhamnosus GG. Clin. Exp. Allergy 2000 ; 30 : 1804-8.   DOI
15 Choi MO, Kim BJ, Jo SK, Jung HK, Lee JT, Kim HY, et al. Anti-allergic activities of Castanea crenata inner shell extracts fermented by Lactobacillus bifermentans. Korean J Food Preserv 2013 ; 20(4) : 583-91.   DOI
16 Park HS, Lee HS, Uhm TB. Selection of microorganisms for probiotics and their characterization. J. Korean Soc. Food Sci. Nutr. 1998 ; 27 : 433-40.
17 Klliomaki M, Ouwehand A, Arvilommi H, Kero P, Isolauri E. Transforming growth factor beta in the breastmilk: A potential regulator of atopic disease at an early age. J. Allergy Clin. Immun. 1999 ; 104 : 1251-7.   DOI
18 Lee IH, Lee SH, Lee IS, Park YK, Chung DK, Cho RW. Effects of Probiotic Extracts of Kimchi on Immune Function in NC/Nga Mice. KOREAN J. FOOD. 2008 ; 40(1) : 82-7.
19 Isolauri E, Rautava S, Kalliomaki M, Kirjavainen P, Salminen S. Role of probiotics in food hypersensitivity. Curr Opin Allergy Clin Immunol 2002 ; 2 : 263-71.   DOI
20 Brouwer ML, Wolt-Plompen SAA, Dubois AEJ, Van Der Heide S, Jansen DF, Hoijer MA, et al. No effects of probiotics on atopic dermatitis in infancy: a randomized placebo-controlled trial. Clin Exp Allergy 2006 ; 36 : 899-906.   DOI
21 Gruber C, Wendt M, Sulser C, Lau S, Kulig M, Wahn U, et al. Randomized, placebo-controlled trial of Lactobacillus rhamnosus GG as treatment of atopic dermatitis in infancy. Allergy 2007 ; 62 : 1270-6.   DOI
22 Weston S, Halbert A, Richmond P, Prescott SL. Effects of probiotics on atopic dermatitis: a randomised controlled trial. Arch Dis Child 2005 ; 90 : 892-7.   DOI