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http://dx.doi.org/10.14406/acu.2015.006

Pinus densiflora Gnarl Inhibits Migration through Suppression of Protein Kinase C in C6 Glioma Cells  

Min, Ilguk (Division of Bio Science, College of Science and Technology, Dongguk University)
Lee, Kangpa (Division of Bio Science, College of Science and Technology, Dongguk University)
Chang, Haeryong (Division of Bio Science, College of Science and Technology, Dongguk University)
Moon, Jinyoung (Deptartment of Acupoint, College of Korean Medicine, Dongguk University)
Publication Information
Korean Journal of Acupuncture / v.32, no.2, 2015 , pp. 51-58 More about this Journal
Abstract
Objectives : Pinus densiflora gnarl, called Song-Jeol in Korean medicine, has been used to cure inflammatory diseases such as arthritis. In the present study, we evaluated inhibitory property of Song-Jeol pharmacopuncture(SJ) on C6 glioma cell migration. Methods : To evaluate cell viability on C6 glioma cells of SJ, the viability was assessed by using Ez-cytox assay kit. The cell migration was assessed by wound-healing assay and Boyden chamber assay, respectively. LPS-induced NO productions were determined by using the Griess reagent. The expression of iNOS and protein kinase $C(PKC)-{\alpha}$ were estimated by western blotting assay. Results : In the wound-healing assay and Boyden chamber assay, SJ showed a significant inhibition on serum-induced C6 glioma cell migration. In addition, NO production was decreased by SJ through suppression of iNOS expression in LPS-stimulated C6 glioma cell. Futhermore, LPS-induced protein kinase $C(PKC)-{\alpha}$ expression was effectively inhibited by SJ. Conclusions : These results demonstrated that SJ was useful for the suppression of the C6 glioma cell migration.
Keywords
Pinus densiflora gnarl; migration; iNOS; protein kinase C; C6 glioma cells;
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